DTDP-glucose 4,6-dehydratase

From Proteopedia

RmlB dimer complex with NAD, TDP-D-glucose and sulfate (PDB code 1ker) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Function dTDP-glucose 4,6-dehydratase (RmlB) catalyzes the reversible conversion of dTDP-glucose to dTDP-4-dehydro-6-deoxy-D-glucose. RmlB is the second of four enzymes involved in the dTDP-L-rhamnose pathway[1]. RmlB is involved in the pathway LSP O-antigen biosynthesis which is part of the bacterial outer membrane biogenesis[2]. Structural highlights The RmlB structure is divided into 2 domains. The N-terminal NAD-binding domain which contains the Rossmann fold and the C-terminal sugar nucleotide binding domain[3]. . . .

3D structures of RmlB

Updated on 07-February-2023

1kep, 1oc2 – SsRmlB + NAD + TDP-xylose – Streptococcus suis
1ker – SsRmlB + NAD + TDP-glucose
1ket – SsRmlB + NAD + TDP
1keu – StRmlB + NAD + TDP-glucose – Salmonella typhimurium
1kew – StRmlB + NAD + TDP
1g1a – StRmlB + NAD
1bxk – EcRmlB + NAD – Escherichia coli
1r66, 1r6d – RmlB + NAD + TDP derivative – Streptococcus venezuelae
6bi4 – RmlB + NAD + TDP-sucrose – Bacillus anthracis
5u4q – RmlB + NAD – Klebsiella pneumoniae
6bwl – RmlB + NAD + UDP – Bacillus thuringiensis
8du1 – RmlB + NAD – Elizabethkingia anophelis

References

1. ↑ Allard ST, Giraud MF, Whitfield C, Messner P, Naismith JH. The purification, crystallization and structural elucidation of dTDP-D-glucose 4,6-dehydratase (RmlB), the second enzyme of the dTDP-L-rhamnose synthesis pathway from Salmonella enterica serovar typhimurium. Acta Crystallogr D Biol Crystallogr. 2000 Feb;56(Pt 2):222-5. PMID:10666612
2. ↑ Wang Q, Ding P, Perepelov AV, Xu Y, Wang Y, Knirel YA, Wang L, Feng L. Characterization of the dTDP-D-fucofuranose biosynthetic pathway in Escherichia coli O52. Mol Microbiol. 2008 Dec;70(6):1358-67. doi: 10.1111/j.1365-2958.2008.06449.x., Epub 2008 Oct 30. PMID:19019146 doi:http://dx.doi.org/10.1111/j.1365-2958.2008.06449.x
3. ↑ Allard ST, Beis K, Giraud MF, Hegeman AD, Gross JW, Wilmouth RC, Whitfield C, Graninger M, Messner P, Allen AG, Maskell DJ, Naismith JH. Toward a structural understanding of the dehydratase mechanism. Structure. 2002 Jan;10(1):81-92. PMID:11796113