From Proteopedia
proteopedia linkproteopedia link |
Function
Listeriolysin positive regulatory factor A (PrfA) is a transcriptional regulator in the Gram-positive pathogen Listeria monocytogenes[1]. PrfA binds the cognate inducer glutathione in order to be in its active conformaiton. PrfA is the master regulator of virulence factors which are required for bacterial entry, intracellular replication and cell-to-cell spread[2]. See also Transcriptional activator.
Relevance
PrfA is the major virulence regulator of L. monocytogenesis. The bacterium is a pathogen which mainly affecrs elderly, immunocompromised people and pregnant women. It can lead to meningoencephalitis, septicaemia and abortion.
Structural highlights
|
3D structures of listeriolysin positive regulatory factor A
1omi - LmPrfA - Listeria monocytogenes
6qw2, 5qvz, 6qw1, 2bgc, 6qvy - LmPrfA (mutant)
5x6d, 5lej - LmPrfA + DNA
6qwm, 6qwf, 6qwh, 6qwk, 5lek - LmPrfA (mutant) + DNA
5lrr - LmPrfA + glutathione
5x6e, 5lrs - LmPrfA + DNA + glutathione
2beo - LmPrfA (mutant) + Gln
6euz, 6euu, 6exl, 6exm, 6ev0, 6eut, 6exk, 5f1r - LmPrfA + inhibitor
6hck - LmPrfA + dipeptide
References
- ↑ Hansen S, Hall M, Grundstrom C, Brannstrom K, Sauer-Eriksson AE, Johansson J. A novel growth based selection strategy identifies new constitutively active variants of the major virulence regulator PrfA in Listeria monocytogenes. J Bacteriol. 2020 Mar 16. pii: JB.00115-20. doi: 10.1128/JB.00115-20. PMID:32179627 doi:http://dx.doi.org/10.1128/JB.00115-20
- ↑ Sternkopf Lillebaek EM, Lambert Nielsen S, Scheel Thomasen R, Faergeman NJ, Kallipolitis BH. Antimicrobial medium- and long-chain free fatty acids prevent PrfA-dependent activation of virulence genes in Listeria monocytogenes. Res Microbiol. 2017 Jul - Aug;168(6):547-557. doi: 10.1016/j.resmic.2017.03.002. , Epub 2017 Mar 23. PMID:28344104 doi:http://dx.doi.org/10.1016/j.resmic.2017.03.002
