Molecular Playground/ClpP

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Here, in the Chien lab of the University of Massachusetts-Amherst, we study how proteolysis plays a large part in protein quality control. The maintenance and timely destruction of protein levels plays an important role during cell homeostasis and cell transitions/differentiation, yet much of what governs these processes has to be fully understood.


E. coli ClpP protease complex with MPD (PDB code 1yg6).

References

1. ClpP: A structurally dynamic protease regulated by AAA+ proteins. Alexopoulos JA et. al (2012 J Struct Bio)

2. ClpA and ClpX ATPases bind simultaneously to opposite ends of ClpP peptidase to form active hybrid complexes. Ortega J et. al (2004 J. Struct Biol.)

3. Binding of the ClpA Unfoldase Opens the Axial Gate of ClpP Peptidase. Effantin et. al (2010 J Biol Chem)

4. Turned on for degradation: ATPase-independent degradation by ClpP. Bewley MC et. al (2009 J Struct Biol)

5. Control of peptide product sizes by the energy-dependent protease ClpAP. Choi KH et. al (2005 Biochemistry)

6. Clp P represents a unique family of serine proteases. Maurizi MR et. al (1990 J Biolchem)

7. ClpP: a distinctive family of cylindrical energy-dependent serine proteases. Yu AY et. al (2007 FEBS Lett.)

8. Human mitochondrial ClpP is a stable heptamer that assembles into a tetradecamer in the presence of ClpX. Kang SG et. al (2007 J Biol Chem)

9. The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system. Gottesman S et. al (1998 Genes Dev.)

10. Bacterial Cell Stress Protein ClpP: A Novel Antibiotic Target. Brötz-Oesterhelt et. al (2013 Heat Shock Proteins Volume 7, pp 375-385)

11. Homology in structural organization between E. coli ClpAP protease and the eukaryotic 26 S proteasome. Kessel M et. al (1995 J Mol Bio)

12. Proteasomes and their kin: proteases in the machine age. Pickart CM et. al (2004 Nat Rev Mol Cell Biol.)


Acknowledgements

Kamal Joshi, Joanne Lau, Jing Liu, Rob Vass, Lisa Hernandez

PDB id:1YG6 from Bewley, MC et. al (2006 J.Struct.Biol.)

Proteopedia Page Contributors and Editors (what is this?)

Robert Vass, Lisa Hernandez, Michal Harel

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