Nucleolin

From Proteopedia

Hamster nucleolin RNA recognition motifs 1-2 (blue) complex with RNA (green) (PDB code 1rkj) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Function Nucleolin (NCL) is a multifunctional phosphoprotein found in the nucleolus, nucleus and cytoplasm. NCL contains intrinsic DNA and RNA helicase, nucleic-acid-dependent ATPase and self-cleaving activities. NCL binds RNA through its RNA recognition motifs (RBD). It regulates various aspects of DNA and RNA metabolism[1]. Relevance NCL expression is enhanced on the surface of many different types of cancer cells and hence is the target of anti-cancer therapies[2]. NCL is a specific marker of angiogenic endothelial cells in tumor vessels[3]. Structural highlights The interacting with both NCL domains[4]. .

3D structures of nucleolin

Updated on 28-November-2021

2krr – hNCL RBD1-2 – human - NMR
2fc8, 2fc9 – hNCL RRM_1 domain - NMR
1rkj, 1fje – haNCL RBD1-2 + RNA – hamster - NMR
1fj7 – haNCL RBD1 - NMR
1fjc – haNCL RBD2 - NMR

References

1. ↑ Tajrishi MM, Tuteja R, Tuteja N. Nucleolin: The most abundant multifunctional phosphoprotein of nucleolus. Commun Integr Biol. 2011 May;4(3):267-75. doi: 10.4161/cib.4.3.14884. PMID:21980556 doi:http://dx.doi.org/10.4161/cib.4.3.14884
2. ↑ Koutsioumpa M, Papadimitriou E. Cell surface nucleolin as a target for anti-cancer therapies. Recent Pat Anticancer Drug Discov. 2014 May;9(2):137-52. PMID:24251811
3. ↑ Christian S, Pilch J, Akerman ME, Porkka K, Laakkonen P, Ruoslahti E. Nucleolin expressed at the cell surface is a marker of endothelial cells in angiogenic blood vessels. J Cell Biol. 2003 Nov 24;163(4):871-8. PMID:14638862 doi:http://dx.doi.org/10.1083/jcb.200304132
4. ↑ Johansson C, Finger LD, Trantirek L, Mueller TD, Kim S, Laird-Offringa IA, Feigon J. Solution structure of the complex formed by the two N-terminal RNA-binding domains of nucleolin and a pre-rRNA target. J Mol Biol. 2004 Apr 2;337(4):799-816. PMID:15033352 doi:http://dx.doi.org/10.1016/j.jmb.2004.01.056