User:Daniel Seeman/Alpha-1-antitrypsin

From Proteopedia

PDB ID 1atu1ezxwd.pdb Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
1atu, resolution 2.70Å ()
Structural annotation: Resources: CATH : 1Atu002, 1Atu001 InterPro : Ipr000215 Pfam : PF00079 SCOP : d1atu__ UniProt : P01009
Functional annotation: Cellular component: extracellular space extracellular region Biological process: acute-phase response Molecular function: protein binding serine-type endopeptidase inhibitor activity endopeptidase inhibitor activity
Evolutionary conservation: Toggle Conservation Colors: Rows = identical sequences: Further Information: Caveats, Explanation, Complete Results at ConSurfDB
Resources:	FirstGlance, OCA, RCSB, PDBsum
Coordinates:	save as pdb, mmCIF, xml

Alpha-1-antitrypsin (also known as α1-antitrypsin or A1AT) is an inhibitor of Elastase and Trypsin. It is a member of the Serine Protease Inhibitor (Serpin) family, and as such undergoes a conformational change where the substrate protein associates with a loop region on A1AT causing that loop to become ordered as a Beta Strand^[1]. In this case Trypsin (the substrate) is inhibited when a covalent bond is formed to A1AT through the newly formed Beta region^[1]. Once bound covalently to its substrate the stability of the A1AT complex goes up drastically, making it an effective "molecular mousetrap"^[1]. With A1AT, as with most members of the Serpin family, the transition from inactive precursor protein to active complex comes after a cleavage event^[1]. Shown is a morph, generated by the Yale Morph Server that shows A1AT going from its inactive form, to the conformation in which it is bound to Trypsin (also shown in the same animation)^[2].

Role in disease

Mutations of MET358 to ARG can lead to a change in specificity in the Elastase binding pocket, essentially turning the M358R mutant of A1AT into a Thrombin inhibitor by generating specificity for this new substrate. This drop in Thrombin levels can lead to hemorrhaging. ^[3]

A mutation in the genes coding for A1AT known as the "Z-antitrypsin mutation" can lead to formation of an inactive polymer made up of subunits of this protein. This polymer builds up in liver cell endoplasmic reticulums leading to cirrhosis of the liver and emphysema. One explanation for this is that a decrease in the flexibility of A1AT is preventing it from inserting its loop region into the proper region of the same subunit. This model suggests that the loop region of one subunit of A1AT inserts into the middle of another subunit, leading to polymerization. However this method does not explain the excess stability of the complex. An alternative model suggests that this polymerization is the result of domain swapping^[1].

Scenes

See Also

• 1atu
• 1ezx

References

1. ↑ ^{1.0 1.1 1.2 1.3 1.4} Nature 455, 1189-1190 (30 October 2008)
2. ↑ The Yale Morph Server
3. ↑ Biochemistry, Fifth Edition, p.289.