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|3e0j, resolution 3.00Å ()|
X-ray structure of the complex of regulatory subunits of human DNA polymerase delta
The eukaryotic DNA polymerase delta (Pol delta) participates in genome replication, homologous recombination, DNA repair and damage tolerance. Regulation of the plethora of Pol delta functions depends on the interaction between the second (p50) and third (p66) non-catalytic subunits. We report the crystal structure of p50*p66(N) complex featuring oligonucleotide binding and phosphodiesterase domains in p50 and winged helix-turn-helix N-terminal domain in p66. Disruption of the interaction between the yeast orthologs of p50 and p66 by strategic amino acid changes leads to cold-sensitivity, sensitivity to hydroxyurea and to reduced UV mutagenesis, mimicking the phenotypes of strains where the third subunit of Pol delta is absent. The second subunits of all B family replicative DNA polymerases in archaea and eukaryotes, except Pol delta, share a three-domain structure similar to p50*p66(N), raising the possibility that a portion of the gene encoding p66 was derived from the second subunit gene relatively late in evolution.
X-ray structure of the complex of regulatory subunits of human DNA polymerase delta., Baranovskiy AG, Babayeva ND, Liston VG, Rogozin IB, Koonin EV, Pavlov YI, Vassylyev DG, Tahirov TH, Cell Cycle. 2008 Oct;7(19):3026-36. Epub 2008 Oct 4. PMID:18818516
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
- Baranovskiy AG, Babayeva ND, Liston VG, Rogozin IB, Koonin EV, Pavlov YI, Vassylyev DG, Tahirov TH. X-ray structure of the complex of regulatory subunits of human DNA polymerase delta. Cell Cycle. 2008 Oct;7(19):3026-36. Epub 2008 Oct 4. PMID:18818516
- Baranovskiy AG, Babayeva ND, Pavlov YI, Tahirov TH. Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol delta. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Sep 1;64(Pt, 9):822-4. Epub 2008 Aug 20. PMID:18765914 doi:10.1107/S1744309108025086