2qo3

Crystal Structure of [KS3][AT3] didomain from module 3 of 6-deoxyerthronolide B synthase

spinqualitylabelsall models 2qo3, resolution 2.59Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2qo3 is a 2 chain structure with sequence from Saccharopolyspora erythraea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.59Å Ligands: ACT, CER, CL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ERYA2_SACER Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed We report the 2.6 A X-ray crystal structure of a 190 kDa homodimeric fragment from module 3 of the 6-deoxyerthronolide B synthase covalently bound to the inhibitor cerulenin. The structure shows two well-organized interdomain linker regions in addition to the full-length ketosynthase (KS) and acyltransferase (AT) domains. Analysis of the substrate-binding site of the KS domain suggests that a loop region at the homodimer interface influences KS substrate specificity. We also describe a model for the interaction of the catalytic domains with the acyl carrier protein (ACP) domain. The ACP is proposed to dock within a deep cleft between the KS and AT domains, with interactions that span both the KS homodimer and AT domain. In conjunction with other recent data, our results provide atomic resolution pictures of several catalytically relevant protein interactions in this remarkable family of modular megasynthases. Structural and mechanistic analysis of protein interactions in module 3 of the 6-deoxyerythronolide B synthase.,Tang Y, Chen AY, Kim CY, Cane DE, Khosla C Chem Biol. 2007 Aug;14(8):931-43. PMID:17719492 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also 6-deoxyerythronolide B synthase 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA