2yjl

Structural characterization of a secretin pilot protein from the type III secretion system (T3SS) of Pseudomonas aeruginosa

spinqualitylabelsall models 2yjl, resolution 1.81Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2yjl is a 3 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.81Å Ligands: NI, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function EXSB_PSEAE Publication Abstract from PubMed Pseudomonas aeruginosa is an opportunistic human pathogen that employs a finely tuned type III secretion system (T3SS) to inject toxins directly into the cytoplasm of target cells. ExsB is a 15.6-kDa protein encoded in a T3SS transcription regulation operon that displays high sequence similarity to YscW, a lipoprotein from Yersinia spp. whose genetic neighborhood also involves a transcriptional regulator, and has been shown to play a role in the stabilization of the outer membrane ring of the T3SS. Here, we show that ExsB is expressed in P. aeruginosa upon induction of the T3SS, and subcellular fractionation studies reveal that it is associated with the outer membrane. The high-resolution crystal structure of ExsB shows that it displays a compact beta-sandwich fold with interdependent beta-sheets. ExsB possesses a large patch of basic residues that could play a role in membrane recognition, and its structure is distinct from that of MxiM, a lipoprotein involved in secretin stabilization in Shigella, as well as from those of Pil lipoproteins involved in pilus biogenesis. These results reveal that small lipoproteins involved in formation of the outer membrane secretin ring display clear structural differences that may be related to the different functions they play in these systems. Structural Characterization and Membrane Localization of ExsB from the Type III Secretion System (T3SS) of Pseudomonas aeruginosa.,Izore T, Perdu C, Job V, Attree I, Faudry E, Dessen A J Mol Biol. 2011 Oct 14;413(1):236-46. Epub 2011 Aug 4. PMID:21839744 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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