3j7z

Structure of the E. coli 50S subunit with ErmCL nascent chain

Loading... Status: waiting for Mol* initialization Structural highlights 3j7z is a 10 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.9Å Ligands: ERY Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RL32_ECOLI Publication Abstract from PubMed During protein synthesis, nascent polypeptide chains within the ribosomal tunnel can act in cis to induce ribosome stalling and regulate expression of downstream genes. The Staphylococcus aureus ErmCL leader peptide induces stalling in the presence of clinically important macrolide antibiotics, such as erythromycin, leading to the induction of the downstream macrolide resistance methyltransferase ErmC. Here, we present a cryo-electron microscopy (EM) structure of the erythromycin-dependent ErmCL-stalled ribosome at 3.9 A resolution. The structure reveals how the ErmCL nascent chain directly senses the presence of the tunnel-bound drug and thereby induces allosteric conformational rearrangements at the peptidyltransferase center (PTC) of the ribosome. ErmCL-induced perturbations of the PTC prevent stable binding and accommodation of the aminoacyl-tRNA at the A-site, leading to inhibition of peptide bond formation and translation arrest. Drug Sensing by the Ribosome Induces Translational Arrest via Active Site Perturbation.,Arenz S, Meydan S, Starosta AL, Berninghausen O, Beckmann R, Vazquez-Laslop N, Wilson DN Mol Cell. 2014 Oct 1. pii: S1097-2765(14)00746-1. doi:, 10.1016/j.molcel.2014.09.014. PMID:25306253 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Transfer RNA (tRNA)

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