3vwj

Ternary crystal structure of the human NKT TCR-CD1d-C20:2 complex

spinqualitylabelsall models 3vwj, resolution 3.09Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3vwj is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.093Å Ligands: DB3, MG, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CD1D_HUMAN Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells. Publication Abstract from PubMed Human and mouse type I NKT cells respond to a variety of CD1d-restricted glycolipid antigens (Ags), with their NKT cell antigen receptors (NKT TCRs) exhibiting reciprocal cross-species reactivity that is underpinned by a conserved NKT TCR-CD1d-Ag docking mode. Within this common docking footprint, the NKT TCR recognises, to varying degrees of affinity, a range of Ags. Presently it is unclear whether the human NKT TCRs will mirror the generalities underpinning the fine specificity of the mouse NKT TCR-CD1d-Ag interaction. Here, we assessed human NKT TCR recognition against altered glycolipid ligands of alpha-galactosylceramide (alpha-GalCer), and have determined the structures of a human NKT TCR in complex with CD1d-4',4-deoxy-alpha-GalCer and CD1d-alpha-GalCer with a shorter, di-unsaturated acyl chain (C20:2). AGLs with acyl-chain modifications did not affect the affinity of the human NKT TCR-CD1d-Ag interaction. Surprisingly, human NKT TCR recognition is more tolerant to modifications at the 4'-OH position in comparison to the 3'-OH position of alpha-GalCer, which contrasts the fine specificity of the mouse NKT TCR-CD1d-Ag recognition (4'-OH > 3'-OH). The fine specificity differences between human and mouse NKT TCRs was attributable to differing interactions between the respective complementarity determining region (CDR) 1alpha loops and the Ag. Accordingly, germline encoded fine-specificity differences underpin human and mouse type I NKT TCR interactions, which is an important considerations for therapeutic development and NKT cell physiology. Human and mouse type I Natural Killer T-cell antigen receptors exhibit different fine specificities for CD1d-antigen.,Wun KS, Ross F, Patel O, Besra GS, Porcelli SA, Richardson SK, Keshipeddy S, Howell AR, Godfrey DI, Rossjohn J J Biol Chem. 2012 Sep 20. PMID:22995911 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Beta-2 microglobulin 3D structures CD1 T-cell receptor 3D structures

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