4ang

Small RNA phage PRR1 in complex with an RNA operator fragment

spinqualitylabelsall models 4ang, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ang is a 5 chain structure with sequence from Pseudomonas phage PRR1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.5Å Ligands: CA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function COAT_BPPRR Forms the phage shell; binds to the phage RNA. Publication Abstract from PubMed In small RNA bacteriophages, the genomic RNA binds to the coat proteins when the viral capsid assembles. This is achieved through sequence-specific interactions between a coat-protein dimer and an RNA stem-loop that includes the start codon for the replicase gene. The structure of virus-like particles of the small RNA phage PRR1 bound to an RNA segment corresponding to this stem-loop has been solved and the binding was compared with the related, and better investigated, phage MS2. The overall conformation of the RNA is found to be similar and the residues that are involved in RNA binding in PRR1 are the same as in MS2. The arrangement of the nucleotide bases in the loop of the stem-loop is different, leading to a difference in the stacking at the conserved Tyr86, which is equivalent to Tyr85 in MS2. PRR1 coat protein binding to its RNA translational operator.,Persson M, Tars K, Liljas L Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):367-72. doi:, 10.1107/S0907444912047464. Epub 2013 Feb 16. PMID:23519411 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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