4bq2

Structural analysis of an exo-beta-agarase

spinqualitylabelsall models 4bq2, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4bq2 is a 4 chain structure with sequence from Saccharophagus degradans 2-40. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.9Å Ligands: CA, GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q21HC5_SACD2 Publication Abstract from PubMed The bacteria that metabolize agarose use multiple enzymes of complementary specificities to hydrolyze the glycosidic linkages in agarose, a linear polymer comprising the repeating disaccharide subunit of neoagarobiose (3,6-anhydro-L-galactose-alpha-(1,3)-D-galactose), which are beta-(1,4)-linked. Here we present the crystal structure of a glycoside hydrolase family 50 exo-beta-agarase Aga50D from the marine microbe Saccharophagus degradans. This enzyme catalyzes a critical step in the metabolism of agarose by S. degradans through cleaving agarose oligomers into neoagarobiose products that can be further processed into monomers. The crystal structure of Aga50D to 1.9 &{Aring] resolution reveals a (beta/alpha)8-barrel fold that is elaborated with a beta-sandwich domain and extensive loops. The structure of catalytically inactivated Aga50D in complex with non-hydrolyzed neoagarotetraose (2.05 &{Aring] resolution) and neoagarooctaose (2.30 &{Aring] resolution) provide views of Michaelis complexes for a beta-agarase. In these structures, the D-galactose residue in the -1 subsite is distorted into a 1S3 skew boat conformation. The relative positioning of the putative catalytic residues are most consistent with a retaining catalytic mechanism. Additionally, the neoagarooctaose complex showed that this extended substrate made substantial interactions with the beta-sandwich domain, which resembles a carbohydrate binding module, thus creating additional plus (+) subsites and funneling the polymeric substrate through the tunnel shaped active site. A synthesis of these results in combination with an additional neoagarobiose product complex suggests a potential exo-processive mode of action of Aga50D on the agarose double helix. Substrate recognition and hydrolysis by a family 50 exo-beta-agarase Aga50D from the marine bacterium Saccharophagus degradans.,Pluvinage B, Hehemann JH, Boraston AB J Biol Chem. 2013 Aug 6. PMID:23921382 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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