4ozt

crystal structure of the ligand binding domains of the Bovicola ovis ecdysone receptor EcR/USP heterodimer (PonA crystal)

spinqualitylabelsall models 4ozt, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ozt is a 2 chain structure with sequence from Pediculus humanus corporis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.7Å Ligands: NEQ, P1A Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function E0VVT4_PEDHC Publication Abstract from PubMed The heterodimeric ligand-binding region of the Bovicola ovis ecdysone receptor has been crystallized either in the presence of an ecdysteroid or a synthetic methylene lactam insecticide. Two X-ray crystallographic structures, determined at 2.7 A resolution, show that the ligand-binding domains of both subunits of this receptor, like those of other nuclear receptors, can display significant conformational flexibility. Thermal melt experiments show that while ponasterone A stabilizes the higher order structure of the heterodimer in solution, the methylene lactam destabilizes it. The conformations of the EcR and USP subunits observed in the structure crystallized in the presence of the methylene lactam have not been seen previously in any ecdysone receptor structure and represent a new level of conformational flexibility for these important receptors. Interestingly, the new USP conformation presents an open, unoccupied ligand-binding pocket. Unprecedented conformational flexibility revealed in the ligand-binding domains of the Bovicola ovis ecdysone receptor (EcR) and ultraspiracle (USP) subunits.,Ren B, Peat TS, Streltsov VA, Pollard M, Fernley R, Grusovin J, Seabrook S, Pilling P, Phan T, Lu L, Lovrecz GO, Graham LD, Hill RJ Acta Crystallogr D Biol Crystallogr. 2014 Jul 1;70(Pt 7):1954-64. doi:, 10.1107/S1399004714009626. Epub 2014 Jun 29. PMID:25004972 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Retinoid X receptor 3D structures

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