4rdx

Structure of histidinyl-tRNA synthetase in complex with tRNA(His)

spinqualitylabelsall models 4rdx, resolution 2.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4rdx is a 2 chain structure with sequence from Thermus thermophilus HB27. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.55Å Ligands: AMP, GTP, HIS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SYH_THET2 Publication Abstract from PubMed Aminoacyl-tRNA synthetases (aaRSs) play a crucial role in protein translation by linking tRNAs with cognate amino acids. Among all the tRNAs, only tRNA(His) bears a guanine base at position -1 (G-1), and it serves as a major recognition element for histidyl-tRNA synthetase (HisRS). Despite strong interests in the histidylation mechanism, the tRNA recognition and aminoacylation details are not fully understood. We herein present the 2.55 A crystal structure of HisRS complexed with tRNA(His), which reveals that G-1 recognition is principally nonspecific interactions on this base and is made possible by an enlarged binding pocket consisting of conserved glycines. The anticodon triplet makes additional specific contacts with the enzyme but the rest of the loop is flexible. Based on the crystallographic and biochemical studies, we inferred that the uniqueness of histidylation system originates from the enlarged binding pocket (for the extra base G-1) on HisRS absent in other aaRSs, and this structural complementarity between the 5' extremity of tRNA and enzyme is probably a result of coevolution of both. Structural basis for recognition of G-1-containing tRNA by histidyl-tRNA synthetase.,Tian Q, Wang C, Liu Y, Xie W Nucleic Acids Res. 2015 Mar 11;43(5):2980-90. doi: 10.1093/nar/gkv129. Epub 2015 , Feb 26. PMID:25722375 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Aminoacyl tRNA synthetase 3D structures Transfer RNA (tRNA)

Proteopedia Page Contributors and Editors (what is this?)

OCA