4v4j

Interactions and Dynamics of the Shine-Dalgarno Helix in the 70S Ribosome.

spinqualitylabelsall models 4v4j, resolution 3.83Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4v4j is a 10 chain structure with sequence from Thermus thermophilus HB27. This structure supersedes the now removed PDB entries 1vsp and 2qnh. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.83Å Ligands: 2MG, 5MC, 7MG, M2G, MA6, PSU Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RL1_THET2 Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release (By similarity). Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA (By similarity). Publication Abstract from PubMed The crystal structure of an initiation-like 70S ribosome complex containing an 8-bp Shine-Dalgarno (SD) helix was determined at 3.8-A resolution. Translation-libration-screw analysis showed that the inherent anisotropic motions of the SD helix were biased along its helical axis, suggesting that during the first step of translocation, the SD helix moves along its helical screw axis. Contacts between the SD helix and the ribosome were primarily through interactions with helices 23a, 26, and 28 of 16S rRNA. Contact with the neck (helix 28) of the 30S subunit near its hinge point suggests that formation of the SD helix could affect positioning of the head of the 30S subunit for optimal interaction with initiator tRNA. The bulged U723 in helix 23a interacts with the minor groove of the SD helix at the C1539.G-10 base pair, explaining its selective conservation in bacteria and archaea. Interactions and dynamics of the Shine Dalgarno helix in the 70S ribosome.,Korostelev A, Trakhanov S, Asahara H, Laurberg M, Lancaster L, Noller HF Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):16840-3. Epub 2007 Oct 16. PMID:17940016 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosomal protein THX 3D structures Ribosome 3D structures Transfer RNA (tRNA)

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