4v6k

Structural insights into cognate vs. near-cognate discrimination during decoding.

Loading... Status: waiting for Mol* initialization Structural highlights 4v6k is a 11 chain structure with sequence from Escherichia coli, Escherichia coli DH1 and Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 8.25Å Ligands: 1MG, 2MA, 2MG, 3AU, 3TD, 4OC, 4SU, 5MC, 5MU, 6MZ, 7MG, CH, H2U, MA6, MIA, OMC, OMG, OMU, PSU, UR3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RL34_ECOLI Publication Abstract from PubMed The structural basis of the tRNA selection process is investigated by cryo-electron microscopy of ribosomes programmed with UGA codons and incubated with ternary complex (TC) containing the near-cognate Trp-tRNA(Trp) in the presence of kirromycin. Going through more than 350 000 images and employing image classification procedures, we find approximately 8% in which the TC is bound to the ribosome. The reconstructed 3D map provides a means to characterize the arrangement of the near-cognate aa-tRNA with respect to elongation factor Tu (EF-Tu) and the ribosome, as well as the domain movements of the ribosome. One of the interesting findings is that near-cognate tRNA's acceptor stem region is flexible and CCA end becomes disordered. The data bring direct structural insights into the induced-fit mechanism of decoding by the ribosome, as the analysis of the interactions between small and large ribosomal subunit, aa-tRNA and EF-Tu and comparison with the cognate case (UGG codon) offers clues on how the conformational signals conveyed to the GTPase differ in the two cases. Structural insights into cognate versus near-cognate discrimination during decoding.,Agirrezabala X, Schreiner E, Trabuco LG, Lei J, Ortiz-Meoz RF, Schulten K, Green R, Frank J EMBO J. 2011 Mar 4. PMID:21378755 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Elongation factor 3D structures Ribosome 3D structures Transfer RNA (tRNA)

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