4v7p

Recognition of the amber stop codon by release factor RF1.

spinqualitylabelsall models 4v7p, resolution 3.62Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4v7p is a 20 chain structure with sequence from Thermus thermophilus HB27. This structure supersedes the now removed PDB entries 3mr8, 3mrz, 3ms0 and 3ms1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.62Å Ligands: MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS2_THET2 Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit (By similarity). Publication Abstract from PubMed We report the crystal structure of a termination complex containing release factor RF1 bound to the 70S ribosome in response to an amber (UAG) codon at 3.6-A resolution. The amber codon is recognized in the 30S subunit-decoding centre directly by conserved elements of domain 2 of RF1, including T186 of the PVT motif. Together with earlier structures, the mechanisms of recognition of all three stop codons by release factors RF1 and RF2 can now be described. Our structure confirms that the backbone amide of Q230 of the universally conserved GGQ motif is positioned to contribute directly to the catalysis of the peptidyl-tRNA hydrolysis reaction through stabilization of the leaving group and/or transition state. We also observe synthetic-negative interactions between mutations in the switch loop of RF1 and in helix 69 of 23S rRNA, revealing that these structural features interact functionally in the termination process. These findings are consistent with our proposal that structural rearrangements of RF1 and RF2 are critical to accurate translation termination. Recognition of the amber UAG stop codon by release factor RF1.,Korostelev A, Zhu J, Asahara H, Noller HF EMBO J. 2010 Aug 4;29(15):2577-85. Epub 2010 Jun 29. PMID:20588254 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosomal protein THX 3D structures Ribosome 3D structures Transfer RNA (tRNA)

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