4v8o

Crystal structure of the hybrid state of ribosome in complex with the guanosine triphosphatase release factor 3

spinqualitylabelsall models 4v8o, resolution 3.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4v8o is a 10 chain structure with sequence from Thermus thermophilus HB8. This structure supersedes the now removed PDB entries 3zvo and 3zvp. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.8Å Ligands: GCP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS2_THET8 Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B] Publication Abstract from PubMed Protein release factor 3 (RF3), a guanosine triphosphatase, binds to ribosome after release of the nascent peptide and promotes dissociation of the class I release factors during the termination of protein synthesis. Here we present the crystal structure of the 70S ribosome with RF3 in the presence of a nonhydrolyzable GTP analogue, guanosine 5'-beta,gamma-methylenetriphosphate (GDPCP), refined to 3.8 A resolution. The structure shows that the subunits of the ribosome are rotated relative to each other compared to the canonical state, resulting in a P/E hybrid state for the transfer RNA. The substantial conformational rearrangements in the complex are described and suggest how RF3, by stabilizing the hybrid state of the ribosome, facilitates the dissociation of class I release factors. Crystal structure of the hybrid state of ribosome in complex with the guanosine triphosphatase release factor 3.,Jin H, Kelley AC, Ramakrishnan V Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15798-803. Epub 2011 Sep 8. PMID:21903932 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosomal protein THX 3D structures Ribosome 3D structures Transfer RNA (tRNA)

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