4v95

Crystal structure of YAEJ bound to the 70S ribosome

spinqualitylabelsall models 4v95, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4v95 is a 20 chain structure with sequence from Thermus thermophilus HB8. This structure supersedes the now removed PDB entries 4dh9, 4dha, 4dhb and 4dhc. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RL32_THET8 Found on the solvent side of the large subunit.[HAMAP-Rule:MF_00340] Publication Abstract from PubMed In bacteria, the hybrid transfer-messenger RNA (tmRNA) rescues ribosomes stalled on defective messenger RNAs (mRNAs). However, certain gram-negative bacteria have evolved proteins that are capable of rescuing stalled ribosomes in a tmRNA-independent manner. Here, we report a 3.2 angstrom-resolution crystal structure of the rescue factor YaeJ bound to the Thermus thermophilus 70S ribosome in complex with the initiator tRNA(i)(fMet) and a short mRNA. The structure reveals that the C-terminal tail of YaeJ functions as a sensor to discriminate between stalled and actively translating ribosomes by binding in the mRNA entry channel downstream of the A site between the head and shoulder of the 30S subunit. This allows the N-terminal globular domain to sample different conformations, so that its conserved GGQ motif is optimally positioned to catalyze the hydrolysis of peptidyl-tRNA. This structure gives insights into the mechanism of YaeJ function and provides a basis for understanding how it rescues stalled ribosomes. Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome.,Gagnon MG, Seetharaman SV, Bulkley D, Steitz TA Science. 2012 Mar 16;335(6074):1370-2. PMID:22422986 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures Transfer RNA (tRNA)

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