4v9c

Allosteric control of the ribosome by small-molecule antibiotics

spinqualitylabelsall models 4v9c, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4v9c is a 20 chain structure with sequence from Escherichia coli K-12. This structure supersedes the now removed PDB entries 4gaq, 4gar, 4gas and 4gau. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG, NMY, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS2_ECOLI Publication Abstract from PubMed Protein synthesis is targeted by numerous, chemically distinct antibiotics that bind and inhibit key functional centers of the ribosome. Using single-molecule imaging and X-ray crystallography, we show that the aminoglycoside neomycin blocks aminoacyl-transfer RNA (aa-tRNA) selection and translocation as well as ribosome recycling by binding to helix 69 (H69) of 23S ribosomal RNA within the large subunit of the Escherichia coli ribosome. There, neomycin prevents the remodeling of intersubunit bridges that normally accompanies the process of subunit rotation to stabilize a partially rotated ribosome configuration in which peptidyl (P)-site tRNA is constrained in a previously unidentified hybrid position. Direct measurements show that this neomycin-stabilized intermediate is incompatible with the translation factor binding that is required for distinct protein synthesis reactions. These findings reveal the functional importance of reversible intersubunit rotation to the translation mechanism and shed new light on the allosteric control of ribosome functions by small-molecule antibiotics. Allosteric control of the ribosome by small-molecule antibiotics.,Wang L, Pulk A, Wasserman MR, Feldman MB, Altman RB, Doudna Cate JH, Blanchard SC Nat Struct Mol Biol. 2012 Aug 19. doi: 10.1038/nsmb.2360. PMID:22902368 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures Ribosome recycling factor Transfer RNA (tRNA)

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