4v9n

Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2.

spinqualitylabelsall models 4v9n, resolution 3.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4v9n is a 20 chain structure with sequence from Thermus thermophilus HB27. This structure supersedes the now removed PDB entries 4kfh, 4kfi, 4kfk and 4kfl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.4Å Ligands: MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q72GJ6_THET2 Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (By similarity). Publication Abstract from PubMed Bacterial translation termination is mediated by release factors RF1 and RF2, which recognize stop codons and catalyze hydrolysis of the peptidyl-tRNA ester bond. The catalytic mechanism has been debated. We proposed that the backbone amide NH group, rather than the side chain, of the glutamine of the universally conserved GGQ motif participates in catalysis by H-bonding to the tetrahedral transition-state intermediate and by product stabilization. This was supported by complete loss of RF1 catalytic activity when glutamine is replaced by proline, the only residue that lacks a backbone NH group. Here, we present the 3.4 A crystal structure of the ribosome complex containing the RF2 Q253P mutant and find that its fold, including the GGP sequence, is virtually identical to that of wild-type RF2. This rules out proline-induced misfolding and further supports the proposal that catalytic activity requires interaction of the Gln-253 backbone amide with the 3' end of peptidyl-tRNA. Crystal Structure of the 70S Ribosome Bound with the Q253P Mutant Form of Release Factor RF2.,Santos N, Zhu J, Donohue JP, Korostelev AA, Noller HF Structure. 2013 Jun 12. pii: S0969-2126(13)00158-5. doi:, 10.1016/j.str.2013.04.028. PMID:23769667 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosomal protein THX 3D structures Ribosome 3D structures Transfer RNA (tRNA)

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