5c8j

A YidC-like protein in the archaeal plasma membrane

spinqualitylabelsall models 5c8j, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5c8j is a 12 chain structure with sequence from Homo sapiens and Methanocaldococcus jannaschii DSM 2661. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.502Å Ligands: LMT Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Y480_METJA Publication Abstract from PubMed Cells possess specialized machinery to direct the insertion of membrane proteins into the lipid bilayer. In bacteria, the essential protein YidC inserts certain proteins into the plasma membrane, and eukaryotic orthologs are present in the mitochondrial inner membrane and the chloroplast thylakoid membrane. The existence of homologous insertases in archaea has been proposed based on phylogenetic analysis. However, limited sequence identity, distinct architecture, and the absence of experimental data have made this assignment ambiguous. Here we describe the 3.5-A crystal structure of an archaeal DUF106 protein from Methanocaldococcus jannaschii (Mj0480), revealing a lipid-exposed hydrophilic surface presented by a conserved YidC-like fold. Functional analysis reveals selective binding of Mj0480 to ribosomes displaying a stalled YidC substrate, and a direct interaction between the buried hydrophilic surface of Mj0480 and the nascent chain. These data provide direct experimental evidence that the archaeal DUF106 proteins are YidC/Oxa1/Alb3-like insertases of the archaeal plasma membrane. A YidC-like Protein in the Archaeal Plasma Membrane.,Borowska MT, Dominik PK, Anghel SA, Kossiakoff AA, Keenan RJ Structure. 2015 Aug 5. pii: S0969-2126(15)00278-6. doi:, 10.1016/j.str.2015.06.025. PMID:26256539 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Antibody 3D structures 3D structures of human antibody

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