5j6h

Recognition of the MHC class Ib molecule H2-Q10 by the natural killer cell receptor Ly49C

spinqualitylabelsall models 5j6h, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5j6h is a 3 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Ligands: NA, NCA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function HA10_MOUSE Involved in the presentation of foreign antigens to the immune system. Publication Abstract from PubMed Murine Natural Killer (NK) cells are regulated by the interaction of Ly49 receptors with major histocompatibility complex class I (MHC-I) molecules. While the ligands for inhibitory Ly49 were considered to be restricted to classical MHC (MHC-Ia), we have shown that the non-classical MHC (MHC-Ib) molecule H2-M3 was a ligand for the inhibitory Ly49A. Here we establish that another MHC-Ib molecule, H2-Q10, is a bona fide ligand for the inhibitory Ly49C receptor. H2-Q10 bound to Ly49C with a lower affinity (~ 5muM) than that observed between Ly49C and MHC-Ia (H-2Kb/H-2Dd, both ~ 1muM) and this recognition could be prevented by cis interactions with H-2Kb in situ. In order to understand the molecular details underpinning Ly49/MHC-Ib recognition, we determined the crystal structures of H2-Q10 and Ly49C bound H2-Q10. Unliganded H2-Q10 adopted a classical MHC-I fold and possessed a peptide-binding groove that exhibited features similar to those found in MHC-Ia, explaining the diverse peptide-binding repertoire of H2-Q10. Ly49C bound to H2-Q10 underneath the peptide-binding platform to a region that encompassed residues from the alpha1, alpha2 and alpha3 domains, as well as the associated beta2-microglobulin subunit. This docking mode was conserved with that previously observed for Ly49C/H-2Kb. Indeed, structure-guided mutation of Ly49C indicated that Ly49C/H2-Q10 and Ly49C/H-2Kb possess similar energetic footprints focused around residues located within the Ly49C beta4-stand and L5 loop, which contact the underside of the peptide binding platform floor. Our data provides a structural basis for Ly49-MHC-Ib recognition and demonstrate that MHC-Ib represent an extended family of ligands for Ly49 molecules. Recognition of the MHC class Ib molecule H2-Q10 by the natural killer cell receptor Ly49C.,Sullivan LC, Berry R, Sosnin N, Widjaja JM, Deuss FA, Balaji GR, LaGruta NL, Mirams M, Trapani JA, Rossjohn J, Brooks AG, Andrews DM J Biol Chem. 2016 Jul 6. pii: jbc.M116.737130. PMID:27385590 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Beta-2 microglobulin 3D structures MHC 3D structures MHC I 3D structures

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