6d7k

Complex structure of Methane monooxygenase hydroxylase in complex with inhibitory subunit

spinqualitylabelsall models 6d7k, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6d7k is a 8 chain structure with sequence from Methylosinus sporium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.6Å Ligands: FE, FMT, HEZ Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q27RN6_METSR Publication Abstract from PubMed Soluble methane monooxygenase in methanotrophs converts methane to methanol under ambient conditions. The maximum catalytic activity of hydroxylase (MMOH) is achieved through the interplay of its regulatory protein (MMOB) and reductase. An additional auxiliary protein, MMOD, functions as an inhibitor of MMOH; however, its inhibitory mechanism remains unknown. Here, we report the crystal structure of the MMOH-MMOD complex from Methylosinus sporium strain 5 (2.6 A). Its structure illustrates that MMOD associates with the canyon region of MMOH where MMOB binds. Although MMOD and MMOB recognize the same binding site, each binding component triggers different conformational changes toward MMOH, which then respectively lead to the inhibition and activation of MMOH. Particularly, MMOD binding perturbs the di-iron geometry by inducing two major MMOH conformational changes, i.e., MMOH beta subunit disorganization and subsequent His(147) dissociation with Fe1 coordination. Furthermore, 1,6-hexanediol, a mimic of the products of sMMO, reveals the substrate access route. MMOD-induced structural changes of hydroxylase in soluble methane monooxygenase.,Kim H, An S, Park YR, Jang H, Yoo H, Park SH, Lee SJ, Cho US Sci Adv. 2019 Oct 2;5(10):eaax0059. doi: 10.1126/sciadv.aax0059. eCollection 2019, Oct. PMID:31616787 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Methane monooxygenase 3D structures

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