6dzm

Bundibugyo virus GP (mucin-deleted) in complex with pan-ebolavirus human antibody ADI-15878 Fab

Loading... Status: waiting for Mol* initialization Structural highlights 6dzm is a 12 chain structure with sequence from Bundibugyo ebolavirus and Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.29Å Ligands: BMA, MAN, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B8XCN0_9MONO Publication Abstract from PubMed Monoclonal antibodies (mAbs) with pan-ebolavirus cross-reactivity are highly desirable, but development of such mAbs is limited by a lack of a molecular understanding of cross-reactive epitopes. The antibody ADI-15878 was previously identified from a human survivor of Ebola virus Makona variant (EBOV/Mak) infection. This mAb demonstrated potent neutralizing activity against all known ebolaviruses and provided protection in rodent and ferret models against three ebolavirus species. Here, we describe the unliganded crystal structure of ADI-15878 as well as the cryo-EM structures of ADI-15878 in complex with the EBOV/Mak and Bundibugyo virus (BDBV) glycoproteins (GPs). ADI-15878 binds through an induced-fit mechanism by targeting highly conserved residues in the internal fusion loop (IFL), bridging across GP protomers via the heptad repeat 1 (HR1) region. Our structures provide a more complete description of the ebolavirus immunogenic landscape, as well as a molecular basis for how rare but potent antibodies target conserved filoviral fusion machinery. Structural Basis of Pan-Ebolavirus Neutralization by an Antibody Targeting the Glycoprotein Fusion Loop.,Murin CD, Bruhn JF, Bornholdt ZA, Copps J, Stanfield R, Ward AB Cell Rep. 2018 Sep 4;24(10):2723-2732.e4. doi: 10.1016/j.celrep.2018.08.009. PMID:30184505 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glycoprotein GP 3D structures Monoclonal Antibodies 3D structures 3D structures of human antibody

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