6ht6

Crystal structure of glutathione transferase Omega 2S from Trametes versicolor in complex with 2,4-dihydroxybenzophenone

spinqualitylabelsall models 6ht6, resolution 2.67Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ht6 is a 2 chain structure with sequence from Trametes versicolor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.671Å Ligands: CUT, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A452CSY5_TRAVE Publication Abstract from PubMed Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases is especially expanded in lignolytic fungi, little is known about their target molecules. In this study, by combining biochemical, enzymatic and structural approaches, interactions between polyphenols and six glutathione transferases from the white-rot fungus Trametes versicolor have been demonstrated. Two isoforms, named TvGSTO3S and TvGSTO6S have been deeply studied at the structural level. Each isoform shows two distinct ligand-binding sites, a narrow L-site at the dimer interface and a peculiar deep hydrophobic H-site. In TvGSTO3S, the latter appears optimized for aromatic ligand binding such as hydroxybenzophenones. Affinity crystallography revealed that this H-site retains the flavonoid dihydrowogonin from a partially purified wild-cherry extract. Besides, TvGSTO6S binds two molecules of the flavonoid naringenin in the L-site. These data suggest that TvGSTO isoforms could interact with plant polyphenols released during wood degradation. Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor.,Schwartz M, Perrot T, Aubert E, Dumarcay S, Favier F, Gerardin P, Morel-Rouhier M, Mulliert G, Saiag F, Didierjean C, Gelhaye E Sci Rep. 2018 May 31;8(1):8472. doi: 10.1038/s41598-018-26601-3. PMID:29855494 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glutathione S-transferase 3D structures

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