6lrd

Structure of RecJ complexed with a 5'-P-dSpacer-modified ssDNA

spinqualitylabelsall models 6lrd, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6lrd is a 3 chain structure with sequence from Deinococcus radiodurans and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.901335Å Ligands: 3DR, MN, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D0EM60_DEIRD Publication Abstract from PubMed RecJ reportedly participates in the base excision repair (BER) pathway, but structural and functional data are scarce. Herein, the Deinococcus radiodurans RecJ (drRecJ) deletion strain exhibited extreme sensitivity to hydrogen peroxide and methyl-methanesulphonate, as well as a high spontaneous mutation rate and an accumulation of unrepaired abasic sites in vivo, indicating the involvement of drRecJ in the BER pathway. The binding affinity and nuclease activity preference of drRecJ toward DNA substrates containing a 5'-P-dSpacer group, a 5'-deoxyribose-phosphate (dRP) mimic, were established. A 1.9 A structure of drRecJ in complex with 5'-P-dSpacer-modified single-stranded DNA (ssDNA) revealed a 5'-monophosphate binding pocket and occupancy of 5'-dRP in the drRecJ nuclease core. The mechanism for RecJ 5'-dRP catalysis was explored using structural and biochemical data, and the results implied that drRecJ is not a canonical 5'-dRP lyase. Furthermore, in vitro reconstitution assays indicated that drRecJ tends to participate in the long-patch BER pathway rather than the short-patch BER pathway. Participation of RecJ in the base excision repair pathway of Deinococcus radiodurans.,Cheng K, Xu Y, Chen X, Lu H, He Y, Wang L, Hua Y Nucleic Acids Res. 2020 Sep 1. pii: 5900117. doi: 10.1093/nar/gkaa714. PMID:32870272 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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