7djc

Crystal structure of the G26C/Q250A mutant of LeuT

spinqualitylabelsall models 7djc, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7djc is a 1 chain structure with sequence from Aquifex aeolicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.701Å Ligands: BOG, LEU, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O67854_AQUAE Publication Abstract from PubMed The neurotransmitter:sodium symporter (NSS) homolog LeuT from Aquifex aeolicus has proven to be a valuable model for studying the transport mechanism of the NSS family. Crystal structures have captured LeuT in key conformations visited during the transport cycle, allowing for the construction of a nearly complete model of transport, with much of the conformational dynamics studied by computational simulations. Here, we report crystal structures of LeuT representing new intermediate conformations between the outward-facing open and occluded states. These structures, combined with binding and accessibility studies, reveal details of conformational dynamics that can follow substrate binding at the central substrate-binding site (S1) of LeuT in outward-facing states, suggesting a potential competition for direction between the outward-open and outward-occluded states at this stage during substrate transport. Our structures further support an intimate interplay between the protonation state of Glu290 and binding of Na1 that may ultimately regulate the outward-open-to-occluded transition. Crystal structures of LeuT reveal conformational dynamics in the outward-facing states.,Fan J, Xiao Y, Quick M, Yang Y, Sun Z, Javitch JA, Zhou X J Biol Chem. 2021 Apr 1:100609. doi: 10.1016/j.jbc.2021.100609. PMID:33811858 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Symporter 3D structures

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