7e1b

Crystal structure of VbrR-DNA complex

spinqualitylabelsall models 7e1b, resolution 4.59Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7e1b is a 12 chain structure with sequence from Vibrio parahaemolyticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 4.587Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q87HP4_VIBPA Publication Abstract from PubMed Two-component systems typically consist of a paired histidine kinase and response regulator and couple environmental changes to adaptive responses. The response regulator VbrR from Vibrio parahaemolyticus, a member of the OmpR/PhoB family, regulates virulence and antibiotic resistance genes. The activation mechanism of VbrR remains unclear. Here, we report the crystal structures of full-length VbrR in complex with DNA in the active conformation and the N-terminal receiver domain (RD) and the C-terminal DNA-binding domain (DBD) in both active and inactive conformations. Structural and biochemical analyses suggest that unphosphorylated VbrR adopts mainly as inactive dimers through the DBD at the autoinhibitory state. The RD undergoes a monomer-to-dimer transition upon phosphorylation, which further induces the transition of DBD from an autoinhibitory dimer to an active dimer and enables its binding with target DNA. Our study suggests a new model for phosphorylation-induced activation of response regulators and sheds light on the pathogenesis of V. parahaemolyticus. Structural basis of phosphorylation-induced activation of the response regulator VbrR.,Hong S, Guo J, Zhang X, Zhou X, Zhang P, Yu F Acta Biochim Biophys Sin (Shanghai). 2023 Jan 25;55(1):43-50. doi: , 10.3724/abbs.2022200. PMID:36647726 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Response regulator 3D structure

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