7s0g

Isoproterenol bound beta1 adrenergic receptor in complex with heterotrimeric Gi/s chimera protein

spinqualitylabelsall models 7s0g, resolution 3.86Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7s0g is a 4 chain structure with sequence from Bos taurus, Meleagris gallopavo and Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.86Å Ligands: 5FW Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ADRB1_MELGA Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Publication Abstract from PubMed The beta(1)-adrenergic receptor (beta(1)-AR) can activate two families of G proteins. When coupled to Gs, beta(1)-AR increases cardiac output, and coupling to Gi leads to decreased responsiveness in myocardial infarction. By comparative structural analysis of turkey beta(1)-AR complexed with either Gi or Gs, we investigate how a single G-protein-coupled receptor simultaneously signals through two G proteins. We find that, although the critical receptor-interacting C-terminal alpha5-helices on Galpha(i) and Galpha(s) interact similarly with beta(1)-AR, the overall interacting modes between beta(1)-AR and G proteins vary substantially. Functional studies reveal the importance of the differing interactions and provide evidence that the activation efficacy of G proteins by beta(1)-AR is determined by the entire three-dimensional interaction surface, including intracellular loops 2 and 4 (ICL2 and ICL4). Structural basis and mechanism of activation of two different families of G proteins by the same GPCR.,Alegre KO, Paknejad N, Su M, Lou JS, Huang J, Jordan KD, Eng ET, Meyerson JR, Hite RK, Huang XY Nat Struct Mol Biol. 2021 Nov;28(11):936-944. doi: 10.1038/s41594-021-00679-2. , Epub 2021 Nov 10. PMID:34759376 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Adrenergic receptor 3D structures Transducin 3D structures

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