7u2a
Cryo-electron microscopy structure of human mt-SerRS in complex with mt-tRNA (GCU)
Structural highlights
DiseaseSYSM_HUMAN Hyperuricemia-pulmonary hypertension-renal failure-alkalosis syndrome. The disease is caused by variants affecting the gene represented in this entry. FunctionSYSM_HUMAN Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).[UniProtKB:Q9N0F3] Publication Abstract from PubMedHuman mitochondrial gene expression relies on the specific recognition and aminoacylation of mitochondrial tRNAs (mtRNAs) by nuclear-encoded mitochondrial aminoacyl-tRNA synthetases (mt-aaRSs). Despite their essential role in cellular energy homeostasis, strong mutation pressure and genetic drift have led to an unparalleled sequence erosion of animal mtRNAs. The structural and functional consequences of this erosion are not understood. Here, we present cryo-EM structures of the human mitochondrial seryl-tRNA synthetase (mSerRS) in complex with mtRNA(Ser(GCU)). These structures reveal a unique mechanism of substrate recognition and aminoacylation. The mtRNA(Ser(GCU)) is highly degenerated, having lost the entire D-arm, tertiary core, and stable L-shaped fold that define canonical tRNAs. Instead, mtRNA(Ser(GCU)) evolved unique structural innovations, including a radically altered T-arm topology that serves as critical identity determinant in an unusual shape-selective readout mechanism by mSerRS. Our results provide a molecular framework to understand the principles of mito-nuclear co-evolution and specialized mechanisms of tRNA recognition in mammalian mitochondrial gene expression. Structural basis for shape-selective recognition and aminoacylation of a D-armless human mitochondrial tRNA.,Kuhle B, Hirschi M, Doerfel LK, Lander GC, Schimmel P Nat Commun. 2022 Aug 30;13(1):5100. doi: 10.1038/s41467-022-32544-1. PMID:36042193[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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