7wkj

A COVID-19 T-cell response detection method based on a newly identified human CD8+ T cell epitope from SARS-CoV-2-Hubei Province, 2021.

spinqualitylabelsall models 7wkj, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7wkj is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A6M6CC39_HUMAN Publication Abstract from PubMed INTRODUCTION: Similar to antibody detection, severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2)-specific T-cell response evaluation is also pivotal among the coronavirus disease 2019 (COVID-19) convalescents and the vaccinated populations. Nucleocapsid (N) protein is one of the main structural proteins of SARS-CoV-2 and can trigger T-cell responses in humans. METHODS: An overlapping peptide pool covering the full length of the N protein was designed, peptides with positive T-cell activating potency in COVID-19 convalescents were screened, and CD8(+) T cell epitopes were further identified. The epitope was used to detect the SARS-CoV-2-specific CD8(+) T cell responses in COVID-19 convalescents based in intracellular cytokine staining and tetramer staining in flow cytometry. RESULTS: A human leukocyte antigen A (HLA-A)*1101-restricted CD8(+) T cell epitope, which could stimulate the production of IFN-gamma via peripheral blood mononuclear cells (PBMCs) of the convalescents was defined, and the tetramer generated with this epitope could detect SARS-CoV-2-specific T cells in the PBMCs of the convalescents. The structural investigation eliminated that the epitope was a typical HLA-A*1101-restricted T-cell epitope which was conserved among all the sarbecoviruses. DISCUSSION: The newly identified SARS-CoV-2-derived T-cell epitope was helpful to detect the cellular immunity against different sarbecoviruses including SARS-CoV and SARS-CoV-2. This study provided an evaluation method and also a peptide candidate for the research and development of T-cell based vaccine for the virus. A COVID-19 T-Cell Response Detection Method Based on a Newly Identified Human CD8(+) T Cell Epitope from SARS-CoV-2 - Hubei Province, China, 2021.,Zhang J, Lu D, Li M, Liu M, Yao S, Zhan J, Liu WJ, Gao GF China CDC Wkly. 2022 Feb 4;4(5):83-87. doi: 10.46234/ccdcw2021.258. PMID:35186375 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Beta-2 microglobulin 3D structures

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