8i85

Crystal structure of Cph001-D189N in complex with ATP

spinqualitylabelsall models 8i85, resolution 2.17Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8i85 is a 2 chain structure with sequence from Streptosporangium roseum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.17Å Ligands: ACT, ATP, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D2B3F1_STRRD Publication Abstract from PubMed Metagenomic-based studies have predicted an extraordinary number of potential antibiotic-resistance genes (ARGs). These ARGs are hidden in various environmental bacteria and may become a latent crisis for antibiotic therapy via horizontal gene transfer. In this study, we focus on a resistance gene cph, which encodes a phosphotransferase (Cph) that confers resistance to the antituberculosis drug capreomycin (CMN). Sequence Similarity Network (SSN) analysis classified 353 Cph homologues into five major clusters, where the proteins in cluster I were found in a broad range of actinobacteria. We examine the function and antibiotics targeted by three putative resistance proteins in cluster I via biochemical and protein structural analysis. Our findings reveal that these three proteins in cluster I confer resistance to CMN, highlighting an important aspect of CMN resistance within this gene family. This study contributes towards understanding the sequence-structure-function relationships of the phosphorylation resistance genes that confer resistance to CMN. Discovery and characterization of genes conferring natural resistance to the antituberculosis antibiotic capreomycin.,Toh SI, Elaine Keisha J, Wang YL, Pan YC, Jhu YH, Hsiao PY, Liao WT, Chen PY, Ko TM, Chang CY Commun Biol. 2023 Dec 19;6(1):1282. doi: 10.1038/s42003-023-05681-6. PMID:38114770 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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