8pok

Cryo-EM structure of cell-free synthesized human histamine H2 receptor coupled to heterotrimeric Gs protein in lipid environment

spinqualitylabelsall models 8pok, resolution 3.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8pok is a 5 chain structure with sequence from Homo sapiens and Lama glama. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.4Å Ligands: HSM Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Here we describe the cryo-electron microscopy structure of the human histamine 2 receptor (H(2)R) in an active conformation with bound histamine and in complex with G(s) heterotrimeric protein at an overall resolution of 3.4 A. The complex was generated by cotranslational insertion of the receptor into preformed nanodisc membranes using cell-free synthesis in E. coli lysates. Structural comparison with the inactive conformation of H(2)R and the inactive and G(q)-coupled active state of H(1)R together with structure-guided functional experiments reveal molecular insights into the specificity of ligand binding and G protein coupling for this receptor family. We demonstrate lipid-modulated folding of cell-free synthesized H(2)R, its agonist-dependent internalization and its interaction with endogenously synthesized H(1)R and H(2)R in HEK293 cells by applying a recently developed nanotransfer technique. Cryo-EM structure of cell-free synthesized human histamine 2 receptor/G(s) complex in nanodisc environment.,Kock Z, Schnelle K, Persechino M, Umbach S, Schihada H, Januliene D, Parey K, Pockes S, Kolb P, Dotsch V, Moller A, Hilger D, Bernhard F Nat Commun. 2024 Feb 28;15(1):1831. doi: 10.1038/s41467-024-46096-z. PMID:38418462 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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