8tq8

Crystal structure of Fab.34.5.8 in complex with MHC-I (H2-Dd)

spinqualitylabelsall models 8tq8, resolution 2.69Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8tq8 is a 10 chain structure with sequence from Human immunodeficiency virus 1 and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.69Å Ligands: EDO, GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed mAbs to MHC class I (MHC-I) molecules have proved to be crucial reagents for tissue typing and fundamental studies of immune recognition. To augment our understanding of epitopic sites seen by a set of anti-MHC-I mAb, we determined X-ray crystal structures of four complexes of anti-MHC-I Fabs bound to peptide/MHC-I/beta2-microglobulin (pMHC-I). An anti-H2-Dd mAb, two anti-MHC-I alpha3 domain mAbs, and an anti-beta2-microglobulin mAb bind pMHC-I at sites consistent with earlier mutational and functional experiments, and the structures explain allelomorph specificity. Comparison of the experimentally determined structures with computationally derived models using AlphaFold Multimer showed that although predictions of the individual pMHC-I heterodimers were quite acceptable, the computational models failed to properly identify the docking sites of the mAb on pMHC-I. The experimental and predicted structures provide insight into strengths and weaknesses of purely computational approaches and suggest areas that merit additional attention. Experimental Structures of Antibody/MHC-I Complexes Reveal Details of Epitopes Overlooked by Computational Prediction.,Boyd LF, Jiang J, Ahmad J, Natarajan K, Margulies DH J Immunol. 2024 Apr 15;212(8):1366-1380. doi: 10.4049/jimmunol.2300839. PMID:38456672 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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