Alkaline phosphatase

spinqualitylabelsall models E. coli alkaline phosphatase dimer with Zn+2 (grey), Mg+2 (green) and phosphate ions, 1elx Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Alkaline phosphatase (ALP) is an enzyme which removes phosphate from nucleotides, proteins and alkaloids. The enzyme is most effective in alkaline environment. Human ALP is present as 3 tissue-associated isozymes: intestinal, tissue-nonspecific and placental (ALPP). ALP is a zinc and magnesium containing enzyme. ALP is a glycoprotein which is attached by a GPI anchor to cell surfaces. See Alkaline phosphatase (Hebrew) Disease High levels of ALP are associated with hyperphosphatasia with mental retardation syndrome. Relevance ALP is used in molecular biology to remove the phosphate at the 5' end of DNA thus preventing its self ligation. ALP activity is used in the dairy industry as a marker for successful pasteurization since it does not denature at temperatures which kill bacteria in milk. Structural highlights The ALP active site includes the Zn+2 and Mg+2 ions. Water molecules are shown as red spheres. Zn cluster. Mg+2 coordination site. 3D Structures of alkaline phosphatase Alkaline phosphatase 3D structures

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