File:Figure 6.jpeg
Original file (1,007 × 1,025 pixels, file size: 108 KB, MIME type: image/jpeg)
Proteopedia Page Contributors and Editors (what is this?)
Summary
This is an edited image of figure 6 in Stein EG, Ghirlando R, Hubbard SR. Structural basis for dimerization of the Grb10 Src homology 2 domain. Implications for ligand specificity. J Biol Chem. 2003 Apr 11;278(15):13257-64. Epub 2003 Jan 27.
As seen in lanes 1 and 2, the BPS-SH2 proteins did not travel down the gel due to their high pI; to resolve this issue, the researchers added IRK_3P to the two BPS-SH2 proteins which then made a complex that was mobile. Lane 4 shows a band labeled 2:2 complex that shows the position of the SH2 dimer. The additional band found at the very top of lane 4 represents the BPS-SH2_WT protein that did not complex with high motility protein IRK_3P, i.e. it was not able to migrate through the gel due to its high pI. Lane 5 shows a band labeled 1:1 complex elucidating that the Arg substitution at Phe515 did indeed produce a monomer, which was able to travel farther down the gel.
Licensing
{{subst:Non-commercial from license selector}}
Proteopedia Page Contributors and Editors (what is this?)
File history
Click on a date/time to view the file as it appeared at that time.
| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 05:29, 8 November 2012 | | 1,007 × 1,025 (108 KB) | Jason Marks (talk | contribs) | This is an edited image of figure 6 in Stein EG, Ghirlando R, Hubbard SR. Structural basis for dimerization of the Grb10 Src homology 2 domain. Implications for ligand specificity. J Biol Chem. 2003 Apr 11;278(15):13257-64. Epub 2003 Jan 27. |
You cannot overwrite this file.
File usage
The following page uses this file:
