Isopropylmalate dehydrogenase

spinqualitylabelsall models 3-isopropylmalate dehydrogenase complex with 3-isopropylmalate, glycerol, NAD, PMSF, Mn+2 and K+ ions (purple) (PDB entry 4f7i) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function 3-Isopropylmalate dehydrogenase (IMDH) catalyzes the oxidative decarboxylation of 3-isopropylmalate (3IPM) to 2-oxo-4-methylvalerate. This reaction is a step in the biosynthesis of leucine in bacteria and fungi. IMDH uses NAD as a cofactor. IMDH is a bifunctional enzyme that catalyzes dehydrogenation and decarboxylation in the presence of NAD and a divalent cation. Structural highlights 3-isopropylmalate dehydrogenase complex with 3-isopropylmalate, glycerol, NAD, PMSF, Mn+2 and K- ions. IMDH active site containing the NAD cofactor is located between 2 subunits and contains the substrate and Mn+2 and K- ions. Water molecules shown as red spheres. MN coordination site. K coordination site. 3-isopropylmalate binding site. Whole IMDH active site.

3D structures of isopropylmalate dehydrogenase

Updated on 30-August-2020

{"openlevels":0}

• 3-isopropylmalate dehydrogenase
  • NAD, 1xaa, 1xab, 1xac, 1xad, 1osi, 1dpz, 1dr0, 1dr8, 2y3z – TtIMDH – Thermus thermophilus
    
  • 1idm, 1osj, 1wal, 1gc8, 1gc9, 1g2u – TtIMDH (mutant)
    
  • 2ayq, 1v53 – BcIMDH – Bacillus coagulans
    
  • 1v5b – BcIMDH (mutant)
    
  • 1cm7 – IMDH – Escherichia coli
    
  • 1vlc – IMDH – Thermotoga maritima
    
  • 1v7l - IMDH – Pyrococcus horikoshii
    
  • 1w0d, 2g4o - MtIMDH - Mycobacterium tuberculosis
    
  • 3h5e, 3h5h, 3h5j - MtIMDH small subunit
    
  • 2hcu – IMDH small subunit – Streptococcus mutans
    
  • 3q3w, 3udu - IMDH small subunit – Campylobacter jejuni
    
  • 3r8w - AtIMDH – Arabidopsis thaliana
    
  • 3u1h - IMDH – Bacillus
    
  • 3vml - SoIMDH – Shewanella oneidensis/Shewanella benthica
    
• IMDH binary complexes
  • 1hex – TtIMDH + NAD
    
  • 2y40 - TtIMDH + Mn
    
  • 1cnz – IMDH + Mn – Salmonella typhimurium
    
  • 1wpw - IMDH + Mg – Sulfolobus tokodaii
    
  • 2pkp - IMDH small subunit + Zn – Methanocaldococcus jannaschii
    
  • 4iwh - BtIMDH + Mg – Burkholderia thailandensis
    
  • 4xxv - BtIMDH + NAD
    
  • 5j33 - AtIMDH + NAD
    
  • 5j34 - AtIMDH + Mg
    
• IMDH ternary complexes
  • 2y42 - TtIMDH + Mn + NAD
    
  • 2ztw - TtIMDH + Mg + inhibitor + NAD
    
  • 2y41 - TtIMDH + 3IPM + Mn
    
  • 4f7i - TtIMDH + Mg + 3IPM + NAD
    
  • 4wuo - TtIMDH (mutant) + Mn + 3IPM + NAD
    
  • 1a05 – IMDH + 3IPM + Mg - Acidithiobacillus ferrooxidans
    
  • 3vkz, 3vl2, 3vl3, 3vl4, 3vl6, 3vl7 - SoIMDH + 3IPM + Ca
    
  • 3vmj, 3wzw, 3wzv - SoIMDH + 3IPM + Mg
    
  • 3wzy, 3wzx - SoIMDH (mutant) + 3IPM + Mg
    
  • 3vmk - SoIMDH + 3IPM + Mg
    
  • 4y1p - IMDH + 3IPM + Mg – Sulfolobus acidarius
    
  • 5j32 - AtIMDH + 3IPM + Mg
    
  • 6xxy - IMDH (mutant) + NAD + isobutenyl oxalylhydroxamate – Haemophilus influenzae
    

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman