NADPH-Cytochrome P450 Reductase

spinqualitylabelsall models Human NADPH-Cytochrome P450 reductase FMN-binding and FAD/NADPH domains complex with FMN, FAD, NADP and Ca+2 ion (green) (PDB entry 3qe2) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function NADPH-Cytochrome P450 Reductase (P450r) or NADPH cytochrome P450 oxidoreductase is the electron donor for several oxygenase enzymes including Cytochrome P450. See also NADPH Cytochrome P450 Oxidoreductase. Disease Mutations in P450r resulting in its deficiency are observed in many steroid metabolizing diseases and in skeletal malformation. Structural highlights P450r electron transfer cofactors: FMN, FAD, NADPH are all in proximity. NADP binding site. Water molecule are shown as red spheres. FAD/NADP connection via Thr538 and water molecule. FAD binding site. FAD and FMN are also in proximity. FMN binding site. NADP and FAD are in pockets, while FMN is in tunnel.

3D Structures of NADPH-Cytochrome P450 Reductase

Updated on 24-March-2026

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• P450r FMN-binding domain (residues 61-241)
  • NADPH Cytochrome P450 Oxidoreductase - hP450r + FMN - human
    
• P450r FAD/NADPH domain (residues 241-677)
  • 3qfs – hP450r + FAD + NADP
    
  • 3qft - hP450r (mutant) + FAD + NADP
    
• P450r FMN-binding and FAD/NADPH domains (residues 64-677)
  • 3qe2, 5fa6 – hP450r + FAD + FMN + NADP
    
  • 3qfc, 3qfr, 5emn – hP450r (mutant) + FAD + FMN + NADP
    
  • 3es9, 1ja0, 5urd, 5ure, 5urg - rP450r + FAD + FMN + NADP - rat
    
  • 1j9z, 1ja1, 4y7c, 4y9r, 4y9u, 5urh, 6njr, 7l18 - rP450r (mutant) + FAD + FMN + NADP
    
  • 4yaf, 4yal - rP450r + FAD + FMN + AMP
    
  • 4yaw, 4yao, 4yau, 5uri - rP450r (mutant) + FAD + FMN + AMP
    
  • 3ojw, 3ojx - rP450r (mutant) + FAD + FMN
    
  • 3wkt - rP450r (mutant) + heme oxygenase 1 + FAD + FMN + NADP + heme
    
  • 1amo - rP450r + FAD + FMN + NADP
    
  • 6njr - rP450r (mutant) + FAD + FMN + NADP + pyrrolydine derivative
    
  • 2bpo - yP450r (mutant) + FAD + FMN + NADP - yeast
    
  • 2bf4, 2bn4 - yP450r + FAD + FMN + NADP
    
  • 3fjo – yP450r/hP450r + FAD + FMN
    
• P450r
  • 9ef0 - rP450r + FAD + FMN – Cryo EM
    
  • 7sux, 7sv0 – sP450r + FAD + NADP - sorghum
    
  • 7suz – sP450r + FAD
    
  • 5ucw - P450r (mutant) – Bacillus megaterium
    
  • 5gxu - P450r + FAD + FMN – Arabidopsis thaliana
    
  • 6t1u - CtP450r + FAD + FMN – Candida tropicalis
    
  • 6t1t - CtP450r + FAD + FMN + NADP
    

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Alexander Berchansky, Michal Harel, Joel L. Sussman