Peptidyl-tRNA hydrolase

spinqualitylabelsall models Structure of peptidyl-tRNA hydrolase complex with tRNA CCA acceptor domain (PDB entry 3vjr) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Peptidyl-tRNA hydrolase (PTH) is a bacterial enzyme which cleaves peptidyl-tRNA to free peptide and tRNA. PTH catalyzes the reaction in cases of aborted translation for recycling the tRNA for further rounds of protein synthesis. PTH is essential for bacterial viability.rew)]]. See also Peptidyl-tRNA hydrolase 2, mitochondrial (Hebrew). Structural highlights PTH dimer interacts with tRNA at the latter's CCA - the 3' end of the molecule which binds the amino acid; the acceptor site which pass-pairs tRNA N-terminal nucleotides to the C-terminal nucleotides and the TΨC site (Ψ is pseudouridine) which is the tRNA ribosome recognition site.

3D Structures of peptidyl-tRNA hydrolase

Updated on 27-April-2026

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• Peptidyl-tRNA hydrolase
  • Peptidyl-tRNA hydrolase 2, mitochondrial (Hebrew) – hPTH residues 63-179– human
    
  • 2pth – EcPTH – Escherichia coli
    
  • 3ofv – EcPTH (mutant)
    
  • 2rtx – EcPTH GGQ domain
    
  • 1xty – PTH – Pyrococcus abyssi
    
  • 1wn2, 2d3k – PTH – Pyrococcus horikoshii
    
  • 2z2i, 2z2j, 2z2k, 3tck, 3tcn, 3td2, 3td6 – MtPTH – Mycobacterium tuberculosis
    
  • 2jrc – MtPTH – NMR
    
  • 3erj – PTH – Archaeoglobus fulgidus
    
  • 2zv3 – PTH – Mechanocaldococcus jannaschii
    
  • 3kjz, 3kk0, 3p2j, 2naf – MsPTH – Mycobacterium smegmatis
    
  • 2lgj – MsPTH – NMR
    
  • 3nea – PTH – Francisella tularensis
    
  • 3v2i – PTH – Burkholderia thailandesis
    
  • 4dhw, 4djj, 4erx, 4fno, 4fyj, 4jc4, 4qaj – PaPTH – Pseudomonas aeruginosa
    
  • 4fop, 4fot, 4hoy, 4iko, 4jy7, 5y98, 4lwq, 5yl8, 5yla, 5yn4, 5zzv, 6a31, 6ivv, 6iye, 6j93, 6jgu, 6jj1, 6jjq, 6jkx, 6jqt, 6jk8, 6kl8, 6lso, 6lsp, 7csn - AbPTH – Acinetobacter baumannii
    
  • 4olj, 6ix6 – AbPTH (mutant)
    
  • 4p7b – PTH – Salmonella enterica
    
  • 2mjl, 4zxp, 5ivp, 5gvz – VcPTH – Vibrio cholerae
    
  • 4z86, 5b6j, 5ekt, 5ike, 5imb, 5zk0 – VcPTH (mutant)
    
  • 4qt4 – PTH – Streptococcus pyogenes
    
  • 4yly – PTH – Streptococcus aureus
    
  • 5zx8 – TtPTH – Thermus thermophilus
    
  • 8x5u – TtPTH (mutant)
    
  • 7brd – PTH – Klebsiella pneumoniae
    
  • 9ls8 – PTH – Enterococcus faecium
    
• Peptidyl-tRNA hydrolase complex
  • 3vjr – EcPTH + tRNA CCA acceptor domain
    
  • 4jwk, 5y9a – AbPTH + cytidine derivative
    
  • 4qd3 – PaPTH + cytidine derivative
    
  • 4jx9 – AbPTH + uridine
    
  • 4qbk – PaPTH + adenosine derivative
    
  • 8axp – PTH + thyazole derivative – Neisseria gonorrhoeae
    
  • 8x5t – TtPTH + AMP
    

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