| Function
Phosphate-binding protein (PBP) binds phosphate (Pi) with high affinity. PBP is involved in various biological processes like cell cycle regulation. PBP is produced under conditions of low Pi concentration. It binds Pi in the periplasm and transfers it to a membrane protein which transports it to the cytoplasm. PBP undergoes conformational change upon binding to Pi[1].
Structural highlights
The PBP phosphate-binding site is highly selective. It can bind monobasic and dibasic phosphate but does not bind sulfate[2].
- ↑ Gonzalez D, Richez M, Bergonzi C, Chabriere E, Elias M. Crystal structure of the phosphate-binding protein (PBP-1) of an ABC-type phosphate transporter from Clostridium perfringens. Sci Rep. 2014 Oct 16;4:6636. doi: 10.1038/srep06636. PMID:25338617 doi:https://dx.doi.org/10.1038/srep06636
- ↑ Wang Z, Choudhary A, Ledvina PS, Quiocho FA. Fine tuning the specificity of the periplasmic phosphate transport receptor. Site-directed mutagenesis, ligand binding, and crystallographic studies. J Biol Chem. 1994 Oct 7;269(40):25091-4. PMID:7929197
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3D structures of phosphate-binding protein
phosphate-binding protein 3D structures
References
