Protein kinase Spk1

spinqualitylabelsall models Structure of yeast Rad53 FHA1 domain (cyan) complex with phosphothreonine peptide (green) (PDB code 1k3n). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Protein kinase Spk1 (Rad53) is a yeast serine/threonine protein kinase which phosphorylates proteins on serine, threonine and tyrosine. Rad53 controls S-phase checkpoint and G1 and G2 DNA damage checkpoints. Structural highlights Rad53 contains phosphothreonine (PTO) recognition domains: FHA1 at the N-terminal (residues 1-164) which selects for Asp at position +3 relative to PTO and FHA2 at the C-terminal (residues 573-730) which selects for Ile at position +3 relative to PTO. Two SCD - SQ/TQ-rich cluster domains – are flanking the kinase domain. SCD domain is associated with DNA-damage-response proteins. The yeast FHA1 domain interacts with peptide containing PTOXXD sequence.

3D structures of protein kinase Spk1

Updated on 03-February-2022

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• Rad53 FHA1 domain 1-164
  • 1k3j – yRad53 – yeast - NMR
    
  • 1g3g, 1j4o – yRad53 (mutant) - NMR
    
  • 1j4p, 1j4q, 1k3n, 1k3q – yRad53 + Rad9 peptide - NMR
    
  • 1g6g, 2a0t – yRad53 + PTO peptide
    
  • 2jqi – yRad53 + PTO peptide - NMR
    
  • 5t2f - yRad53/DBF4 BRCT domain
    
  • 5t2s - yRad53/DBF4 BRCT domain + phosphopeptide
    
• Rad53 FHA2 domain 573-730
  • 1qu5, 1dmz, 1fhq – yRad53 – yeast - NMR
    
  • 1fhr, 1j4k, 1j4l, 1k2m, 1k2n – yRad53 + Rad9 peptide - NMR
    
• Rad53 kinase and SCD2 domains residues 170-512
  • 4pdp – yRad53 kinase and SCD2 domain (mutant)
    
  • 4pds – yRad53 kinase and SCD2 domain (mutant) + AMPPNP
    
• Rad53 residues 1-466
  • 5xzw – yRad53
    
  • 5xzv – yRad53 + AMPPNP
    

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