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Function
Regulatory protein E2 (RPE2) is a human papillomavirus (HPV) protein which is required for its replication. RPE2 targets the helicase Replication protein E1 (RPE1) to the origin of replication (ori). The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites[1]. RPE2 is found also in bovine papillomavirus (BPV).
Disease
RPE2 induces apoptosis of its host cells[2].
Structural highlights
The complex between HPV6a DBD and 18-bp DNA (PDB code 2ayg) shows the binding of 2 recognition helices of the dimer to 2 successive major grooves of the target DNA making numerous contacts to the DNA backbone phosphate atoms and to its bases[3].
- ↑ Van Horn G, Sheikh S, Khan SA. Regulation of human papillomavirus type 1 replication by the viral E2 protein. Virology. 2001 Aug 15;287(1):214-24. doi: 10.1006/viro.2001.1037. PMID:11504556 doi:https://dx.doi.org/10.1006/viro.2001.1037
- ↑ Singh N, Senapati S, Bose K. Insights into the mechanism of human papillomavirus E2-induced procaspase-8 activation and cell death. Sci Rep. 2016 Feb 24;6:21408. doi: 10.1038/srep21408. PMID:26906543 doi:https://dx.doi.org/10.1038/srep21408
- ↑ Hooley E, Fairweather V, Clarke AR, Gaston K, Brady RL. The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein. Nucleic Acids Res. 2006;34(14):3897-908. Epub 2006 Aug 12. PMID:16914454 doi:10.1093/nar/gkl466
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3D structures of regulatory protein E2
Updated on 10-February-2021
Domains: transactivation (TAD) 2-201; DNA-binding (DBD) 286-365
2ayg, 2aye - RPE2 DBD - HPV6a
2ayb, 2ayg - RPE2 DBD + DNA
1r6k, 1r6n - RPE2 TAD + inhibitor - HPV11
1dto - RPE2 TAD - HPV16
2nnu - RPE2 TAD + BRD4 peptide
1by9, 2q79 - RPE2 DBD
3mi7 - RPE2 DBD (mutant)
1r8p, 1zzf - RPE2 DBD - NMR
1tue - RPE2 TAD + RPE1 - HPV18
1qqh - RPE2 TAD
1f9f - RPE2 DBD
1jj4 - RPE2 DBD + DNA
1dhm - RPE2 DBD - HPV31 - NMR
1a7g - RPE2 DBD - HPV32
2jeu, 2jex - RPE2 TAD (mutant) - BPV1
1jjh, 6bus - RPE2 DBD
1dbd - RPE2 DBD - NMR
2bop - RPE2 DBD + DNA
References
