Function
Ribosome biogenesis proteins or ribosome assembly factor are factors which contribute to the maturation of the ribosome.
Interactive 3D figure on right side shows: Ribosome biogenesis protein Nop10 (pink) complexed with pseudouridine synthase (grey), GAR1 (green), ribosomal protein L7AE (yellow), H/ACA RNA (magenta) and RNA containing pseudouridine (cyan) (PDB code 3hay)
- Erb1 Nop7 and Ytm1 are essential for the correct ribosomal RNA processing[1].
- Nop10 is part of the nucleoprotein particle which catalyzes RNA pseudouridylation and direct processing of ribosomal RNA[2].
- Utp30 is a ribosomal L1 domain-containing protein which is part of the 90S preribosome[3].
- Tsr1 is essential for cytoplasmic steps in the 40S ribosomal subunit maturation[4].
- Nep1 is essential in 18S ribosomal RNA procssing[5].
- Bms1 is part of a complex required for the 40S ribosomal subunit maturation[6].
Structural highlights
The Nop10 containing nucleoprotein particle contains the modified pseudouridine which interacts with residues of the pseudouridine synthase[7].
- ↑ Marcin W, Neira JL, Bravo J. The Carboxy-Terminal Domain of Erb1 Is a Seven-Bladed ss-Propeller that Binds RNA. PLoS One. 2015 Apr 16;10(4):e0123463. doi: 10.1371/journal.pone.0123463., eCollection 2015. PMID:25880847 doi:https://dx.doi.org/10.1371/journal.pone.0123463
- ↑ Hamma T, Reichow SL, Varani G, Ferre-D'Amare AR. The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs. Nat Struct Mol Biol. 2005 Dec;12(12):1101-7. Epub 2005 Nov 15. PMID:16286935 doi:10.1038/nsmb1036
- ↑ Hu J, Zhu X, Ye K. Structure and RNA recognition of ribosome assembly factor Utp30. RNA. 2017 Sep 26. pii: rna.062695.117. doi: 10.1261/rna.062695.117. PMID:28951391 doi:https://dx.doi.org/10.1261/rna.062695.117
- ↑ McCaughan UM, Jayachandran U, Shchepachev V, Chen ZA, Rappsilber J, Tollervey D, Cook AG. Pre-40S ribosome biogenesis factor Tsr1 is an inactive structural mimic of translational GTPases. Nat Commun. 2016 Jun 2;7:11789. doi: 10.1038/ncomms11789. PMID:27250689 doi:https://dx.doi.org/10.1038/ncomms11789
- ↑ Taylor AB, Meyer B, Leal BZ, Kotter P, Schirf V, Demeler B, Hart PJ, Entian KD, Wohnert J. The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site. Nucleic Acids Res. 2008 Mar;36(5):1542-54. Epub 2008 Jan 21. PMID:18208838 doi:10.1093/nar/gkm1172
- ↑ Delprato A, Al Kadri Y, Perebaskine N, Monfoulet C, Henry Y, Henras AK, Fribourg S. Crucial role of the Rcl1p-Bms1p interaction for yeast pre-ribosomal RNA processing. Nucleic Acids Res. 2014;42(15):10161-72. doi: 10.1093/nar/gku682. Epub 2014 Jul, 26. PMID:25064857 doi:https://dx.doi.org/10.1093/nar/gku682
- ↑ Liang B, Zhou J, Kahen E, Terns RM, Terns MP, Li H. Structure of a functional ribonucleoprotein pseudouridine synthase bound to a substrate RNA. Nat Struct Mol Biol. 2009 Jul;16(7):740-6. Epub 2009 May 28. PMID:19478803 doi:10.1038/nsmb.1624
