|
Function
Transcription factor tau or TFIIIC is required for transcription of tRNA and 5S RNA genes by RNA polymerase III[1]. TFIIIC conserved complex is involved in the mechanism controlling general genome organization[2]. TFIIIC is a multisubunit protein which initiates transcription complex assembly on tRNA by binding to the internal box B promoter element of RNA polymerase III[3].
Structural highlights
TFIIIC contains 6 subunits which are organized in 2 subcomplexes. Subcomplex A contains the 131 kDa, 95 kDa and 55kDa subunits while subcomplex B contains 138 kDa, 91 kDa and 60 kDa subunits[4]. The structure of TFIIIC subunit SCF1 DNA-binding domain contains a winged-helix domain (colored in green) and displays a basic patch which is the possible DNA-binding surface (colored in royalblue)[5].
- ↑ Dumay-Odelot H, Marck C, Durrieu-Gaillard S, Lefebvre O, Jourdain S, Prochazkova M, Pflieger A, Teichmann M. Identification, molecular cloning, and characterization of the sixth subunit of human transcription factor TFIIIC. J Biol Chem. 2007 Jun 8;282(23):17179-89. doi: 10.1074/jbc.M611542200. Epub 2007 , Apr 4. PMID:17409385 doi:https://dx.doi.org/10.1074/jbc.M611542200
- ↑ Noma K, Cam HP, Maraia RJ, Grewal SI. A role for TFIIIC transcription factor complex in genome organization. Cell. 2006 Jun 2;125(5):859-72. PMID:16751097 doi:10.1016/j.cell.2006.04.028
- ↑ L'Etoile ND, Fahnestock ML, Shen Y, Aebersold R, Berk AJ. Human transcription factor IIIC box B binding subunit. Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1652-6. PMID:8127861
- ↑ Vorlander MK, Jungblut A, Karius K, Baudin F, Grotsch H, Kosinski J, Muller CW. Structure of the TFIIIC subcomplex tauA provides insights into RNA polymerase III pre-initiation complex formation. Nat Commun. 2020 Sep 30;11(1):4905. doi: 10.1038/s41467-020-18707-y. PMID:32999288 doi:https://dx.doi.org/10.1038/s41467-020-18707-y
- ↑ Taylor NM, Baudin F, von Scheven G, Muller CW. RNA polymerase III-specific general transcription factor IIIC contains a heterodimer resembling TFIIF Rap30/Rap74. Nucleic Acids Res. 2013 Aug 5. PMID:23921640 doi:10.1093/nar/gkt664
|
3D structures of transcription factor tau or TFIIIC
Updated on 28-December-2023
Domains: dimerization 1-110; histidine phosphatase 1-272; DNA-binding 186-396; N-terminal TPR array 123-566; central EWH 546-641
4bjj - fyTFIIIC subunits SFC1 + SFC7 dimerization domain - fission yeast
4bji - fyTFIIIC subunit SFC1 DNA-binding domain
2yn0 - yTFIIIC 55 kDa subunit SFC7 histidine phosphatase domain - yeast
5aem, 5aio - yTFIIIC 131 kDa subunit SFC4 N-terminal TPR array
5aim - yTFIIIC 138 kDa subunit SFC3 central EWH domain
6yj6 - yTFIIIC 131 kDa + 95 kDa + 55 kDa subunits – Cryo EM
8ffz - yTFIIIC 138 kDa + 131 kDa + 95 kDa + 60 kDa + 55kDa subunits + TFIIIA + TFIIIB 70 kDa + DNA – Cryo EM
References
