Molecular Playground/Beta-galactosidase

2016-04-01T07:11:33Z

Doug Juers:

One of the [[CBI Molecules]] being studied in the [http://www.umass.edu/cbi/ University of Massachusetts Amherst Chemistry-Biology Interface Program] at UMass Amherst and on display at the [http://www.molecularplayground.org/ Molecular Playground].

'''β-Galactosidase''' is a hydrolase enzyme that catalyzes the hydrolysis of beta- galactosidase into monosaccharides (carbon/energy source). Is encoded by the ''lacZ'' gene of the ''lac'' operon in E.coli. It is a large 120 kDa, >1000 amino acids) protein that forms a tetramer.

It is commonly used as a reporter molecule in genetics and molecular biology. Usually, experiments are designed so that the β-Galactosidase concentration in the cell is a readout for some aspect of a system being studied. When the β-Galactosidase cleaves the substrate o-nitrophenyl-b-D-galactopyraniside (ONPG), o-nitrophenol is released. This compound has a yellow color, and absorbs 420 nm light. To measure β-Galactosidase activity the accumulation of yellow color (increase 420 nm absorbance)/minute is monitored.

{{STRUCTURE_3iap | PDB=3iap | SCENE= }}

<scene name='Judy_Ventura/Sandbox1/All_protein/1'>All Molecule</scene>

<scene name='Judy_Ventura/Sandbox1/4_subunits/1'>Subunits</scene>

==3D structures of beta-galactosidase==

[[Beta-galactosidase]]

Molecular Playground/Beta-galactosidase

2016-04-01T07:11:00Z

Doug Juers:

One of the [[CBI Molecules]] being studied in the [http://www.umass.edu/cbi/ University of Massachusetts Amherst Chemistry-Biology Interface Program] at UMass Amherst and on display at the [http://www.molecularplayground.org/ Molecular Playground].

'''β-Galactosidase''' is a hydrolase enzyme that catalyzes the hydrolysis of beta- galactosidase into monosaccharides (carbon/energy source). Is encoded by the ''lacZ'' gene of the ''lac'' operon in E.coli. It is a large 120 kDa, >1000 amino acids) protein that forms a tetramer.

It is commonly used as a reporter molecule in genetics and molecular biology. Usually, experiments are designed so that the β-Galactosidase concentration in the cell is a readout for some aspect of a system being studied. When the β-Galactosidase cleaves the substrate o-nitrophenyl-b-D-galactopyraniside (ONPG), o-nitrophenol is released. This compound has a yellow color, and absorbs 420 nm light. To measure β-Galactosidase activity the accumulation of yellow color (increase 420 nm absorbance)/minute is monitored.

{{STRUCTURE_3iap | PDB=3iap | SCENE= }}

<scene name='Judy_Ventura/Sandbox1/All_protein/1'>All Molecule</scene>

<scene name='Judy_Ventura/Sandbox1/4_subunits/1'>Subunits</scene>

<Structure load='Insert PDB code or filename here' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />

==3D structures of beta-galactosidase==

[[Beta-galactosidase]]

Proteopedia - User contributions [en]

Molecular Playground/Beta-galactosidase

Molecular Playground/Beta-galactosidase