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https://proteopedia.org/api.php?action=feedcontributions&feedformat=atom&user=Gabriela+Almeida Proteopedia - User contributions [en] 2026-09-25T04:25:06Z User contributions MediaWiki 1.43.8 https://proteopedia.org/index.php?title=CcNiR&diff=1532676 CcNiR 2012-09-06T13:42:39Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The dissimilatory cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction<ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref>:<br /> <br /> NO2− + 8H+ + 6e− → NH4+ + 2H2O<br /> <br /> This reaction is involved in a respiratory process that represents an important branch of the biological nitrogen cycle.<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a)]]<ref>pmid 17139260<br /> </ref>. The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer; the active site<scene name='CcNiR/Heme1/2'>heme 1</scene>) has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose: on one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits <ref>pmid 17139260</ref>. The overall haem arrangement of the NrfHA complex is rather unusal, comprising 28 hemes in a highly compact arrangement.<br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> All but the active centre at the pentahemic subunit NrfA are hexa-coordinated c-type hemes whereas the catalytic heme is bound to a lysine as the fifth coordinate ligand and has the sixth axial position available for substrate binding. NrfA is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532659 CcNiR 2012-09-06T13:34:32Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The dissimilatory cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction<ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref>:<br /> <br /> NO2− + 8H+ + 6e− → NH4+ + 2H2O<br /> <br /> This reaction is involved in a respiratory process that represents an important branch of the biological nitrogen cycle.<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a)]]<ref>pmid 17139260<br /> </ref>. The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer; the active site(<scene name='CcNiR/Heme1/1'>heme1</scene>) has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose: on one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits <ref>pmid 17139260</ref>. The overall haem arrangement of the NrfHA complex is rather unusal, comprising 28 hemes in a highly compact arrangement.<br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> All but the active centre at the pentahemic subunit NrfA are hexa-coordinated c-type hemes whereas the catalytic heme is bound to a lysine as the fifth coordinate ligand and has the sixth axial position available for substrate binding. NrfA is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532656 CcNiR 2012-09-06T13:33:58Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The dissimilatory cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction<ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref>:<br /> <br /> NO2− + 8H+ + 6e− → NH4+ + 2H2O<br /> <br /> This reaction is involved in a respiratory process that represents an important branch of the biological nitrogen cycle.<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a)]]<ref>pmid 17139260<br /> </ref>. The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer; the active site(<scene name='CcNiR/Heme1/1'>heme1</scene>) has been reported as an unusual lysine-coordinated high-spin heme. NrfH <ref>pmid 17139260</ref><br /> is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. The overall haem arrangement of the NrfHA complex is rather unusal, comprising 28 hemes in a highly compact arrangement.<br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> All but the active centre at the pentahemic subunit NrfA are hexa-coordinated c-type hemes whereas the catalytic heme is bound to a lysine as the fifth coordinate ligand and has the sixth axial position available for substrate binding. NrfA is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532651 CcNiR 2012-09-06T13:31:52Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The dissimilatory cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction<ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref>:<br /> <br /> NO2− + 8H+ + 6e− → NH4+ + 2H2O<br /> <br /> This reaction is involved in a respiratory process that represents an important branch of the biological nitrogen cycle.<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a)]]<ref>pmid 17139260<br /> </ref>. The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer; the active site(<scene name='CcNiR/Heme1/1'>heme1</scene>) has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. The overall haem arrangement of the NrfHA complex is rather unusal, comprising 28 hemes in a highly compact arrangement.<br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> All but the active centre at the pentahemic subunit NrfA are hexa-coordinated c-type hemes whereas the catalytic heme is bound to a lysine as the fifth coordinate ligand and has the sixth axial position available for substrate binding. NrfA is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532649 CcNiR 2012-09-06T13:30:46Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The dissimilatory cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction<ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref>:<br /> <br /> NO2− + 8H+ + 6e− → NH4+ + 2H2O<br /> <br /> This reaction is involved in a respiratory process that represents an important branch of the biological nitrogen cycle.<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a)]]<ref>pmid 17139260<br /> </ref>. The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer; the active site(<scene name='CcNiR/Heme1/1'>heme1</scene>) has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. The overall haem arrangement of the NrfHA complex is rather unusal, comprising 28 hemes in a compact arrangement.<br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> All but the active centre at the pentahemic subunit NrfA are hexa-coordinated c-type hemes whereas the catalytic heme is bound to a lysine as the fifth coordinate ligand and has the sixth axial position available for substrate binding. NrfA is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532636 CcNiR 2012-09-06T13:20:17Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction<ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref>:<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a)]]<ref>pmid 17139260<br /> </ref>. The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer: the active site(<scene name='CcNiR/Heme1/1'>heme1</scene>) has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. The overall haem arrangement of the NrfHA complex is rather unusal, comprising 28 hemes in a compact arrangement.<br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532634 CcNiR 2012-09-06T13:19:31Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction<ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref>:<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a]]<ref>pmid 17139260<br /> </ref>). The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer: the active site(<scene name='CcNiR/Heme1/1'>heme1</scene>) has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. The overall haem arrangement of the NrfHA complex is rather unusal, comprising 28 hemes in a compact arrangement<ref>pmid 17139260<br /> </ref>.<br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532633 CcNiR 2012-09-06T13:19:08Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction<ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref>:<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a]]<ref>pmid 17139260<br /> </ref>:). The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer: the active site(<scene name='CcNiR/Heme1/1'>heme1</scene>) has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. The overall haem arrangement of the NrfHA complex is rather unusal, comprising 28 hemes in a compact arrangement<ref>pmid 17139260<br /> </ref>:.<br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532628 CcNiR 2012-09-06T13:17:15Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction:<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a]]). The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer: the active site(<scene name='CcNiR/Heme1/1'>heme1</scene>) has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. The overall haem arrangement of the NrfHA complex is rather unusal, comprising 28 hemes in a compact arrangement.<br /> <br /> REFERENCES:<br /> <br /> <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532627 CcNiR 2012-09-06T13:16:50Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction:<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a]]). The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer: the active site (<scene name='CcNiR/Heme1/1'>heme1</scene>) has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. The overall haem arrangement of the NrfHA complex is rather unusal, comprising 28 hemes in a compact arrangement.<br /> <br /> REFERENCES:<br /> <br /> <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532560 CcNiR 2012-09-06T12:35:14Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction:<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a]]). The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer: the active site (heme 1) has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. The overall haem arrangement of the NrfHA complex is rather unusal, comprising 28 hemes in a compact arrangement.<br /> <br /> REFERENCES:<br /> <br /> <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532558 CcNiR 2012-09-06T12:34:40Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction:<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a]]). The catalytic subunit NrfA is a pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer: the active site (heme 1) has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. The overall haem arrangement of the NrfHA complex is rather unusal comprising 28 hemes in a compact arrangement.<br /> <br /> REFERENCES:<br /> <br /> <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532554 CcNiR 2012-09-06T12:30:26Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits ([[2j7a]]). The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532552 CcNiR 2012-09-06T12:29:40Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits (PDB entry [[2j7a]]). The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532534 CcNiR 2012-09-06T12:19:54Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits. The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/5'>hemes</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532527 CcNiR 2012-09-06T12:14:34Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits. The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/4'>hemes</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532521 CcNiR 2012-09-06T12:10:24Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits. The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/3'>hemes</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532513 CcNiR 2012-09-06T12:05:55Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits. The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/2'>hemes</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532505 CcNiR 2012-09-06T12:03:30Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> <br /> NO2- + 4 H+ + 6 ferricytochrome c = NH4+ + 6 ferrocytochrome c + 2 H2O<br /> <br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits. The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/1'>hemes</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532485 CcNiR 2012-09-06T11:55:18Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits. The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/1'>hemes</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532484 CcNiR 2012-09-06T11:54:44Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits. The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes3/1'>TextToBeDisplayed</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532480 CcNiR 2012-09-06T11:53:23Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits. The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes/3'>hemes</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532447 CcNiR 2012-09-06T11:36:16Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits. The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes/2'>hemes</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532445 CcNiR 2012-09-06T11:35:19Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> The cytochrome 𝑐 nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC 27774 is able to reduce nitrite to ammonia in a six-electron transfer reaction.<br /> The physiological form of the enzyme is believed to be a double trimer of 2 NrfA and 1 NrfH subunits. The catalytic subunit NrfA is a periplasmic membrane-associated pentaheme cytochrome c where the short distances between <scene name='CcNiR/Hemes/1'>hemes</scene> allow a fast and efficient electron transfer. The active site has been reported as an unusual lysine-coordinated high-spin heme. NrfH is a small membrane-bound cytochrome comprising four c-type heme groups and it serves a double purpose. On one hand, it anchors the catalytic subunits to the membrane. On the other hand, it serves as a quinol oxidase, transferring electrons from the quinone pool to the catalytic subunits. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532434 CcNiR 2012-09-06T11:29:40Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 <scene name='CcNiR/Hemes/1'>hemes</scene>. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532429 CcNiR 2012-09-06T11:28:35Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='2j7a' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[2j7a]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 <scene name='CcNiR/Hemes/1'>hemes</scene>. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA4NrfH4 complex that houses 28 hemes. <br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> The overall haem arrangement of the NrfHA complex is quite surprising, as the NrfH haem groups are positioned non-symmetrically with respect to the two NrfA molecules.<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532397 CcNiR 2012-09-06T11:16:12Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='2j7a' size='300' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[2j7a]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bactera Desulfovibrio desulfuricans and Desulfovibrio desulfuricans, the enzyme is purified as a NrfA4NrfH2 complex that houses 28 <scene name='CcNiR/Hemes/1'>hemes</scene>. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532391 CcNiR 2012-09-06T11:11:44Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 <scene name='CcNiR/Hemes/1'>hemes</scene>. <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532390 CcNiR 2012-09-06T11:11:21Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes./ <scene name='CcNiR/Hemes/1'>hemes</scene> <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532389 CcNiR 2012-09-06T11:10:17Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated to perform the reduction of nitrite to ammonium in a six-electron transfer reaction/ <scene name='CcNiR/Hemes/1'>hemes</scene> <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532362 CcNiR 2012-09-06T10:57:45Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated <scene name='CcNiR/Heme/2'>heme</scene>) to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532356 CcNiR 2012-09-06T10:49:27Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated <scene name='CcNiR/Heme/1'>heme</scene>) to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532353 CcNiR 2012-09-06T10:23:46Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (see <scene name='CcNiR/Fe/4'>iron</scene>) to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 17207484</ref> <ref>pmid 20689707</ref> <ref>pmid 17207484</ref><br /> <br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532348 CcNiR 2012-09-06T09:39:47Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (see <scene name='CcNiR/Fe/4'>iron</scene>) to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532346 CcNiR 2012-09-06T09:38:56Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (see <scene name='CcNiR/Fe/3'>iron</scene>) to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532344 CcNiR 2012-09-06T09:38:32Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (see <scene name='CcNiR/Fe/2'>iron</scene>) to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532271 CcNiR 2012-09-06T09:03:55Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (see <scene name='CcNiR/Fe/4'>iron</scene>) to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532268 CcNiR 2012-09-06T09:03:20Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (see <scene name='CcNiR/Fe/4'>TextToBeDisplayed</scene>) to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532251 CcNiR 2012-09-06T08:57:51Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (see <scene name='CcNiR/Fe/2'>heme iron</scene>) to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532247 CcNiR 2012-09-06T08:56:14Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (<scene name='CcNiR/Fe/2'>heme iron</scene>) to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532244 CcNiR 2012-09-06T08:56:04Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (<scene name='CcNiR/Fe/2'>heme iron</scene> to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532242 CcNiR 2012-09-06T08:55:24Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme (see hemes' iron) <scene name='CcNiR/Fe/2'>heme iron</scene> to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <scene name='CcNiR/Fe/2'>heme iron</scene><br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532241 CcNiR 2012-09-06T08:53:40Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <scene name='CcNiR/Fe/2'>heme iron</scene><br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532196 CcNiR 2012-09-06T08:32:05Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> References:<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532190 CcNiR 2012-09-06T08:31:02Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 12618432</ref> <ref>pmid 8798514</ref> <ref>pmid 20689707</ref><br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532187 CcNiR 2012-09-06T08:30:31Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 8798514</ref> <ref>pmid 12618432</ref> <ref>pmid 20689707</ref><br /> <br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532181 CcNiR 2012-09-06T08:28:42Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 8798514</ref> <ref>pmid 12618432</ref><br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532176 CcNiR 2012-09-06T08:26:51Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref> <ref>pmid 8798514</ref><br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at the pentahemic subunit NrfA which is strongly bound to its physiological electron donor, the smaller hydrophobic polypeptide tetrahemic NrfH, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532168 CcNiR 2012-09-06T08:24:56Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 14511372</ref><br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at a pentahemic subunit of 61 kDa which is strongly bound to its physiological electron donor, a smaller hydrophobic polypeptide tetrahemic of 19 kDa, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida https://proteopedia.org/index.php?title=CcNiR&diff=1532160 CcNiR 2012-09-06T08:22:56Z

<p>Gabriela Almeida: </p> <hr /> <div>== Your Heading Here ('ccNiR') ==<br /> <StructureSection load='1oah' size='500' side='left' caption='CYTOCHROME C NITRITE REDUCTASE FROM DESULFOVIBRIO DESULFURICANS ATCC 27774 (PDB entry [[1oah]])' scene=''><br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction <ref>pmid 20188536</ref><br /> <br /> </StructureSection> <br /> <br /> <br /> Cytochrome c nitrite reductase is a multicenter enzyme that uses a five-coordinated heme to perform the reduction of nitrite to ammonium in a six-electron transfer reaction. In the sulfate reducing bacterium Desulfovibrio desulfuricans ATCC 27774, the enzyme is purified as a NrfA2NrfH complex that houses 14 hemes.<br /> <br /> The catalytic reaction occurs at a high-spin (5-coordinated) heme that is located at a pentahemic subunit of 61 kDa which is strongly bound to its physiological electron donor, a smaller hydrophobic polypeptide tetrahemic of 19 kDa, composed of 4 c-types hemes; in vitro, the protein complexes associate each other forming huge aggregates (min. 890 kDa).<br /> <br /> <references/></div>

Gabriela Almeida