Proteopedia - User contributions [en]

Sandbox Reserved 1808

2023-04-26T18:58:52Z

Grant Simpson:

{{Sandbox_Reserved_CHEM351_Spring2023}}
==Lactonase Enzyme Structure==
<StructureSection load=7RIS size='340' side='right' caption='Caption for this structure' scene=''>
This is a default text for your page ''''''. Click above on '''edit this page''' to modify. Be careful with the < and > signs.
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.

== Function of Lactonase Enzyme ==
The organism from which this protein is found is Rhodopsuedomonas plaustris (Rpa3624). The basic function for the lactonase enzyme is to break down valerolactone. Naturally occurring lactones function as metabolic intermediates, signaling molecules, antibiotics, and more.
== Biological Relevance and Broader Implications ==
Lactones are a diverse set of cyclic ester-containing compounds that play many roles in chemical and biological systems. GVL is one such lactone used as a solvent that can be both produced from plant biomass and used to dissolve plant cell walls into sugars and aromatic molecules for subsequent microbial conversion to fuels and chemicals. Even after the majority is removed from the dissolved biomass, the residual GVL can be toxic to microbial fermentation. Residual GVL could also represent a significant carbon and energy source for tolerant microbes that could assimilate this lactone or convert it into other valuable products. The use of GVL as a renewable solvent for plant deconstruction would benefit from the identification of microbial enzymes that can hydrolyze the lactone ring of this compound.
== Important Amino Acids==
The first five amino acids of the amino terminus at physiological pH are MSNVR. The catalytic amino acids are located in the active center and are responsible for accelerating the enzyme-catalyzed reaction. The catalytic amino acids are N123, N172, and S230.
== Structural Highlights ==
Secondary Structure: Consists of six blades of B-propeller, phosphate molecule bound to calcium and sodium ion with a helix attached to it.
Tertiary Structure: Calcium coordination sphere shows the contacts holding the calcium ion in place.
Quaternary structure: Carbonyl oxygen of 2-HQ overlaps with one of the phosphate oxygens.

</StructureSection>
== References ==
<Bingman, C.A., Hall, B.W., Smith, R.W., Fox, B.G., Donohue, T.J. (2022).© 2022 THE AUTHORS. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.DOI: https://doi.org/10.1016/j.jbc.2022.102782

>

Sandbox Reserved 1808

2023-04-26T16:13:03Z

Grant Simpson:

Sandbox Reserved 1808

2023-04-26T15:57:55Z

Grant Simpson:

{{Sandbox_Reserved_CHEM351_Spring2023}}
==Lactonase Enzyme Structure==
<StructureSection load=7RIS size='340' side='right' caption='Caption for this structure' scene=''>
This is a default text for your page ''''''. Click above on '''edit this page''' to modify. Be careful with the < and > signs.
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.

== Function of Lactonase Enzyme ==
The organism from which this protein is found is Rhodopsuedomonas plaustris (Rpa3624). The basic function for the lactonase enzyme is to break down valerolactone.
== Biological Relevance and Broader Implications ==
Lactones are a diverse set of cyclic ester-containing compounds that play many roles in chemical and biological systems. Naturally occurring lactones function as metabolic intermediates, signaling molecules, antibiotics, and more. GVL is one such lactone used as a solvent that can be both produced from plant biomass and used to dissolve plant cell walls into sugars and aromatic molecules for subsequent microbial conversion to fuels and chemicals. Even after the majority is removed from the dissolved biomass, the residual GVL can be toxic to microbial fermentation. Residual GVL could also represent a significant carbon and energy source for tolerant microbes that could assimilate this lactone or convert it into other valuable products. The use of GVL as a renewable solvent for plant deconstruction would benefit from the identification of microbial enzymes that can hydrolyze the lactone ring of this compound.
== Important Amino Acids==
The first five amino acids of the amino terminus at physiological pH are MSNVR. The catalytic amino acids are located in the active center and are responsible for accelerating the enzyme-catalyzed reaction. The catalytic amino acids are N123, N172, and S230.
== Structural Highlights ==
Secondary Structure: Consists of six blades of B-propeller, phosphate molecule bound to calcium and sodium ion with a helix attached to it.
Tertiary Structure: Calcium coordination sphere shows the contacts holding the calcium ion in place.
Quaternary structure: Carbonyl oxygen of 2-HQ overlaps with one of the phosphate oxygens.

</StructureSection>
== References ==
<references/>

Sandbox Reserved 1808

2023-04-26T15:54:24Z

Grant Simpson:

{{Sandbox_Reserved_CHEM351_Spring2023}}
==Lactonase Enzyme Structure==
<StructureSection load=7RIS size='340' side='right' caption='Caption for this structure' scene=''>
This is a default text for your page ''''''. Click above on '''edit this page''' to modify. Be careful with the < and > signs.
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.

== Function of Lactonase Enzyme ==
The organism from which this protein is found is Rhodopsuedomonas plaustris (Rpa3624). The basic function for the lactonase enzyme is to break down valerolactone.
== Biological Relevance and Broader Implications ==
Lactones are a diverse set of cyclic ester-containing compounds that play many roles in chemical and biological systems. Naturally occurring lactones function as metabolic intermediates, signaling molecules, antibiotics, and more. GVL is one such lactone used as a solvent that can be both produced from plant biomass and used to dissolve plant cell walls into sugars and aromatic molecules for subsequent microbial conversion to fuels and chemicals. Even after the majority is removed from the dissolved biomass, the residual GVL can be toxic to microbial fermentation. Residual GVL could also represent a significant carbon and energy source for tolerant microbes that could assimilate this lactone or convert it into other valuable products. The use of GVL as a renewable solvent for plant deconstruction would benefit from the identification of microbial enzymes that can hydrolyze the lactone ring of this compound.
== Important Amino Acids==
The first five amino acids of the amino terminus at physiological pH are MSNVR.
== Structural Highlights ==
Secondary Structure: Consists of six blades of B-propeller, phosphate molecule bound to calcium and sodium ion with a helix attached to it.
Tertiary Structure: Calcium coordination sphere shows the contacts holding the calcium ion in place.
Quaternary structure: Carbonyl oxygen of 2-HQ overlaps with one of the phosphate oxygens.

</StructureSection>
== References ==
<references/>

Sandbox Reserved 1808

2023-04-26T15:52:58Z

Grant Simpson:

{{Sandbox_Reserved_CHEM351_Spring2023}}
==Lactonase Enzyme Structure==
<StructureSection load=7RIS size='340' side='right' caption='Caption for this structure' scene=''>
This is a default text for your page ''''''. Click above on '''edit this page''' to modify. Be careful with the < and > signs.
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.

== Function of Lactonase Enzyme ==
The organism from which this protein is found is Rhodopsuedomonas plaustris (Rpa3624). The basic function for the lactonase enzyme is to break down valerolactone.
== Biological Relevance and Broader Implications ==
Lactones are a diverse set of cyclic ester-containing compounds that play many roles in chemical and biological systems. Naturally occurring lactones function as metabolic intermediates, signaling molecules, antibiotics, and more. GVL is one such lactone used as a solvent that can be both produced from plant biomass and used to dissolve plant cell walls into sugars and aromatic molecules for subsequent microbial conversion to fuels and chemicals. Even after the majority is removed from the dissolved biomass, the residual GVL can be toxic to microbial fermentation. Residual GVL could also represent a significant carbon and energy source for tolerant microbes that could assimilate this lactone or convert it into other valuable products. The use of GVL as a renewable solvent for plant deconstruction would benefit from the identification of microbial enzymes that can hydrolyze the lactone ring of this compound.
== Important Amino Acids==
The first five amino acids of the amino terminus at physiological pH are MSNVR.
== Structural Highlights ==
Secondary Structure: Consists os six blades of B-propeller, phosphate molecule bound to calcium and sodium ion with a helix attached to it.
Tertiary Structure: Calcium coordination sphere shows the contacts holding the calcium ion in place.
Quaternary structure: Carbonyl oxygen of 2-HQ overlaps with one of the phosphate oxygens.

</StructureSection>
== References ==
<references/>

Sandbox Reserved 1808

2023-04-24T14:28:46Z

Grant Simpson:

{{Sandbox_Reserved_CHEM351_Spring2023}}
==Lactonase Enzyme Structure==
<StructureSection load=7RIS size='340' side='right' caption='Caption for this structure' scene=''>
This is a default text for your page ''''''. Click above on '''edit this page''' to modify. Be careful with the < and > signs.
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.

== Function of Lactonase Enzyme ==
The organism from which this protein is found is Rhodopsuedomonas plaustris (Rpa3624). The basic function for the lactonase enzyme is to break down valerolactone.
== Biological Relevance and Broader Implications ==
Lactones are a diverse set of cyclic ester-containing compounds that play many roles in chemical and biological systems. Naturally occurring lactones function as metabolic intermediates, signaling molecules, antibiotics, and more. GVL is one such lactone used as a solvent that can be both produced from plant biomass and used to dissolve plant cell walls into sugars and aromatic molecules for subsequent microbial conversion to fuels and chemicals. Even after the majority is removed from the dissolved biomass, the residual GVL can be toxic to microbial fermentation. Residual GVL could also represent a significant carbon and energy source for tolerant microbes that could assimilate this lactone or convert it into other valuable products. The use of GVL as a renewable solvent for plant deconstruction would benefit from the identification of microbial enzymes that can hydrolyze the lactone ring of this compound.
== Important Amino Acids==
The first five amino acids of the amino terminus at physiological pH are MSNVR.
== Structural Highlights ==

This is a <scene name='95/954105/Test/1'>test image</scene>.

This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

</StructureSection>
== References ==
<references/>

Sandbox Reserved 1808

2023-04-24T14:20:45Z

Grant Simpson:

{{Sandbox_Reserved_CHEM351_Spring2023}}
==Lactonase Enzyme Structure==
<StructureSection load=7RIS size='340' side='right' caption='Caption for this structure' scene=''>
This is a default text for your page ''''''. Click above on '''edit this page''' to modify. Be careful with the < and > signs.
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.

== Function of Lactonase Enzyme ==
The organism from which this protein is found is Rhodopsuedomonas plaustris (Rpa3624). The basic function for the lactonase enzyme is to break down valerolactone.
== Biological Relevance and Broader Implications ==
Lactones are a diverse set of cyclic ester-containing compounds that play many roles in chemical and biological systems. Naturally occurring lactones function as metabolic intermediates, signaling molecules, antibiotics, and more. GVL is one such lactone used as a solvent that can be both produced from plant biomass and used to dissolve plant cell walls into sugars and aromatic molecules for subsequent microbial conversion to fuels and chemicals. Even after the majority is removed from the dissolved biomass, the residual GVL can be toxic to microbial fermentation. Residual GVL could also represent a significant carbon and energy source for tolerant microbes that could assimilate this lactone or convert it into other valuable products. The use of GVL as a renewable solvent for plant deconstruction would benefit from the identification of microbial enzymes that can hydrolyze the lactone ring of this compound.
== Important Amino Acids==
The first five amino acids of the amino terminus at physiological pH are MSNVR.
== Structural Highlights ==

This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

</StructureSection>
== References ==
<references/>

Sandbox Reserved 1808

2023-04-24T13:58:12Z

Grant Simpson:

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2023-04-24T13:50:20Z

Grant Simpson:

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2023-04-24T13:43:17Z

Grant Simpson:

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2023-04-24T13:38:31Z

Grant Simpson:

{{Sandbox_Reserved_CHEM351_Spring2023}}
==Your Heading Here (maybe something like 'Structure')==
<StructureSection load=7RIS size='340' side='right' caption='Caption for this structure' scene=''>
This is a default text for your page ''''''. Click above on '''edit this page''' to modify. Be careful with the < and > signs.
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.

== Function of your protein ==
The organism from which this protein is found is Rhodopsuedomonas plaustris (Rpa3624). The basic function for the lactonase enzyme is to break down valerolactone.
== Biological relevance and broader implications ==

== Important amino acids==

== Structural highlights ==

This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

</StructureSection>
== References ==
<references/>

Sandbox Reserved 1808

2023-04-18T23:51:42Z

Grant Simpson:

{{Sandbox_Reserved_CHEM351_Spring2023}}
==Your Heading Here (maybe something like 'Structure')==
<StructureSection load=7RIS size='340' side='right' caption='Caption for this structure' scene=''>
This is a default text for your page ''''''. Click above on '''edit this page''' to modify. Be careful with the < and > signs.
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.

== Function of your protein ==

== Biological relevance and broader implications ==

== Important amino acids==

== Structural highlights ==

This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

</StructureSection>
== References ==
<references/>