Proteopedia - User contributions [en]

V-ATPase

2012-09-06T14:15:36Z

Gydo van Zundert:

==Introduction==
Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes. V-ATPases are structurally and mechanically related to F- and A-ATPases.<ref>PMID:15473999</ref>

==V-ATPase components==
The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units (a, d, e, c, c' and c") and which acts as a proton translocator from the cytoplasm to the lumen.<ref>PMID:20450191</ref>

ATP hydrolysis occurs at catalytic sites located at the interface of the A and B subunits.

===V1-domain===
<StructureSection load='3a5c' size='250' side='right' caption='V1-domain of V-ATPase of Thermus Thermophilus (PDB entry [[3j0j]])' scene='V-ATPase/V1-part/1'>
The <scene name='V-ATPase/V1-part/1'>V1-domain</scene> consist of the A3B3 complex where the hydrolysis of ATP occurs (<scene name='V-ATPase/V1-part/3'>A is blue, B is red</scene>), subunit C (<scene name='V-ATPase/V1-part/8'>green</scene>), the D-subunit which is the central stalk (<scene name='V-ATPase/V1-part/2'>purple</scene>), subunit E (<scene name='V-ATPase/V1-part/6'>orange</scene>), subunit F (<scene name='V-ATPase/V1-part/4'>yellow</scene>)and subunit G (<scene name='V-ATPase/V1-part/7'>dark green</scene>).
</StructureSection>

===V0-domain===

==Mechanism of rotation==

==V-ATPase structures==

===PDB===

====V1 complex====
[[3j0j]]: Fitted structure of Thermus thermophilus in a 9.7Å resolution cryo-EM map.

[[3a5c]]

[[3a5d]]
====A3B3 complex====
[[3gqb]]

====Subunit C====
[[1r5z]]

[[1u7l]]

[[1v9m]]

====Subunit E====
[[2kz9]]

[[3k5b]]

[[3v6i]]

====Subunit F====
[[2d00]]

====Subunit G====
[[2kwy]]

[[2k88]]

====Subunit H====
[[1ho8]]

====Vo complex====

[[3aou]]

[[2db4]]

[[2bl2]]

[[2cyd]]

====Subunit a====
[[2rpw]]

[[2nvj]]

===EMDB===
[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/5335_summary.html 5335]: 9.7Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1888_summary.html 1888]: 16Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1640_summary.html 1640]: 25Å resolution map of Saccharomyces cerevisiae V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1590_summary.html 1590]: 17Å resolution map of Manduca sexta V-ATPase.

==References==

<references />

V-ATPase

2012-09-06T14:13:38Z

Gydo van Zundert:

==Introduction==
Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes. V-ATPases are structurally and mechanically related to F- and A-ATPases.<ref>PMID:15473999</ref>

==V-ATPase components==
The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units (a, d, e, c, c' and c") and which acts as a proton translocator from the cytoplasm to the lumen.<ref>PMID:20450191</ref>

ATP hydrolysis occurs at catalytic sites located at the interface of the A and B subunits.

===V1-domain===
<StructureSection load='3a5c' size='250' side='right' caption='V1-domain of V-ATPase of Thermus Thermophilus (PDB entry [[3j0j]])' scene='V-ATPase/V1-part/1'>
The <scene name='V-ATPase/V1-part/1'>V1-domain</scene> consist of the A3B3 complex where the hydrolysis of ATP occurs (<scene name='V-ATPase/V1-part/3'>A is blue, B is red</scene>), subunit C (<scene name='V-ATPase/V1-part/8'>green</scene>), the D-subunit which is the central stalk (<scene name='V-ATPase/V1-part/2'>purple</scene>), subunit E (<scene name='V-ATPase/V1-part/6'>orange</scene>), subunit F (<scene name='V-ATPase/V1-part/4'>yellow</scene>)and subunit G (<scene name='V-ATPase/V1-part/7'>dark green</scene>).
</StructureSection>

===V0-domain===

==Mechanism of rotation==

==V-ATPase structures==

===PDB===

====V1 complex====
[[3j0j]]: Fitted structure of Thermus Thermophilus in a 9.7Å resolution cryo-EM map.

[[3a5c]]

[[3a5d]]
====A3B3 complex====
[[3gqb]]

====Subunit C====
[[1r5z]]

[[1u7l]]

[[1v9m]]

====Subunit E====
[[2kz9]]

[[3k5b]]

[[3v6i]]

====Subunit F====
[[2d00]]

====Subunit G====
[[2kwy]]

[[2k88]]

====Subunit H====
[[1ho8]]

====Vo complex====

[[3aou]]

[[2db4]]

[[2bl2]]

[[2cyd]]

====Subunit a====
[[2rpw]]

[[2nvj]]

===EMDB===
[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/5335_summary.html 5335]: 9.7Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1888_summary.html 1888]: 16Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1640_summary.html 1640]: 25Å resolution map of Saccharomyces cerevisiae V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1590_summary.html 1590]: 17Å resolution map of Manduca sexta V-ATPase.

==References==

<references />

V-ATPase

2012-09-06T14:01:11Z

Gydo van Zundert:

==Introduction==
Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes. V-ATPases are structurally and mechanically related to F- and A-ATPases.<ref>PMID:15473999</ref>

==V-ATPase components==
The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units (a, d, e, c, c' and c") and which acts as a proton translocator from the cytoplasm to the lumen.<ref>PMID:20450191</ref>

ATP hydrolysis occurs at catalytic sites located at the interface of the A and B subunits.

===V1-domain===
<StructureSection load='3a5c' size='250' side='right' caption='V1-domain of V-ATPase of Thermus Thermophilus (PDB entry [[3j0j]])' scene='V-ATPase/V1-part/1'>
The <scene name='V-ATPase/V1-part/1'>V1-domain</scene> consist of the A3B3 complex where the hydrolysis of ATP occurs (<scene name='V-ATPase/V1-part/3'>A is blue, B is red</scene>), subunit C (<scene name='V-ATPase/V1-part/5'>green</scene>), the D-subunit which is the central stalk (<scene name='V-ATPase/V1-part/2'>purple</scene>), subunit E (<scene name='V-ATPase/V1-part/6'>orange</scene>), subunit F (<scene name='V-ATPase/V1-part/4'>yellow</scene>)and subunit G (<scene name='V-ATPase/V1-part/7'>dark green</scene>).
</StructureSection>

===V0-domain===

==Mechanism of rotation==

==V-ATPase structures==

===PDB===

====V1 complex====
[[3j0j]]: Fitted structure of Thermus Thermophilus in a 9.7Å resolution cryo-EM map.

[[3a5c]]

[[3a5d]]
====A3B3 complex====
[[3gqb]]

====Subunit C====
[[1r5z]]

[[1u7l]]

[[1v9m]]

====Subunit E====
[[2kz9]]

[[3k5b]]

[[3v6i]]

====Subunit F====
[[2d00]]

====Subunit G====
[[2kwy]]

[[2k88]]

====Subunit H====
[[1ho8]]

====Vo complex====

[[3aou]]

[[2db4]]

[[2bl2]]

[[2cyd]]

====Subunit a====
[[2rpw]]

[[2nvj]]

===EMDB===
[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/5335_summary.html 5335]: 9.7Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1888_summary.html 1888]: 16Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1640_summary.html 1640]: 25Å resolution map of Saccharomyces cerevisiae V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1590_summary.html 1590]: 17Å resolution map of Manduca sexta V-ATPase.

==References==

<references />

V-ATPase

2012-09-06T13:53:51Z

Gydo van Zundert:

==Introduction==
Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes. V-ATPases are structurally and mechanically related to F- and A-ATPases.<ref>PMID:15473999</ref>

==V-ATPase components==
The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units (a, d, e, c, c' and c") and which acts as a proton translocator from the cytoplasm to the lumen.<ref>PMID:20450191</ref>

ATP hydrolysis occurs at catalytic sites located at the interface of the A and B subunits.

===V1-domain===
<StructureSection load='3a5c' size='250' side='right' caption='V1-domain of V-ATPase of Thermus Thermophilus (PDB entry [[3j0j]])' scene='V-ATPase/V1-part/1'>
The <scene name='V-ATPase/V1-part/1'>V1-domain</scene> consist of the A3B3 complex where the hydrolysis of ATP occurs (<scene name='V-ATPase/V1-part/3'>A is blue, B is red</scene>), subunit C (<scene name='V-ATPase/V1-part/5'>green</scene>), the D-subunit which is the central stalk (<scene name='V-ATPase/V1-part/2'>purple</scene>), subunit E (<scene name='V-ATPase/V1-part/6'>orange</scene>), subunit F (<scene name='V-ATPase/V1-part/4'>yellow</scene>)and subunit G (<scene name='V-ATPase/V1-part/7'>dark green</scene>).

</StructureSection>

==Mechanism of rotation==

==V-ATPase structures==

===PDB===

====V1 complex====
[[3j0j]]: Fitted structure of Thermus Thermophilus in a 9.7Å resolution cryo-EM map.

[[3a5c]]

[[3a5d]]
====A3B3 complex====
[[3gqb]]

====Subunit C====
[[1r5z]]

[[1u7l]]

[[1v9m]]

====Subunit E====
[[2kz9]]

[[3k5b]]

[[3v6i]]

====Subunit F====
[[2d00]]

====Subunit G====
[[2kwy]]

[[2k88]]

====Subunit H====
[[1ho8]]

====Vo complex====

[[3aou]]

[[2db4]]

[[2bl2]]

[[2cyd]]

====Subunit a====
[[2rpw]]

[[2nvj]]

===EMDB===
[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/5335_summary.html 5335]: 9.7Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1888_summary.html 1888]: 16Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1640_summary.html 1640]: 25Å resolution map of Saccharomyces cerevisiae V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1590_summary.html 1590]: 17Å resolution map of Manduca sexta V-ATPase.

==References==

<references />

V-ATPase

2012-09-06T12:20:52Z

Gydo van Zundert:

==Introduction==
Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes.

==V-ATPase components==
The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units and which transports the protons.<ref>PMID:20450191</ref>

==Mechanism of rotation==

==V-ATPase structures==

===PDB===

====V1 complex====
[[3j0j]]: Fitted structure of Thermus Thermophilus in a 9.7Å resolution cryo-EM map.

[[3a5c]]

[[3a5d]]
====A3B3 complex====
[[3gqb]]

====Subunit C====
[[1r5z]]

[[1u7l]]

[[1v9m]]

====Subunit E====
[[2kz9]]

[[3k5b]]

[[3v6i]]

====Subunit F====
[[2d00]]

====Subunit G====
[[2kwy]]

[[2k88]]

====Subunit H====
[[1ho8]]

====Vo complex====

[[3aou]]

[[2db4]]

[[2bl2]]

[[2cyd]]

====Subunit a====
[[2rpw]]

[[2nvj]]

===EMDB===
[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/5335_summary.html 5335]: 9.7Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1888_summary.html 1888]: 16Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1640_summary.html 1640]: 25Å resolution map of Saccharomyces cerevisiae V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1590_summary.html 1590]: 17Å resolution map of Manduca sexta V-ATPase.

==References==

<references />

V-ATPase

2012-09-06T12:20:32Z

Gydo van Zundert:

==Introduction==
Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes.

==V-ATPase components==
The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units and which transports the protons.<ref>PMID:20450191</ref>

==Mechanism of rotation==

==V-ATPase structures==

===PDB===

====V1 complex====
[[3j0j]]: Fitted structure of Thermus Thermophilus in a 9.7Å resolution cryo-EM map.

[[3a5c]]

[[3a5d]]
====A3B3 complex====
[[3gqb]]

====Subunit C====
[[1r5z]]

[[1u7l]]

[[1v9m]]

====Subunit E====
[[2kz9]]

[[3k5b]]

[[3v6i]]

====Subunit F====
[[2d00]]

====Subunit G====
[[2kwy]]

[[2k88]]

====Subunit H====
[[1ho8]]

====Vo complex====

[[3aou]]

[[2db4]]

[[2bl2]]

[[2cyd]]

====Subunit a====
[[2rpw]]

[[2nvj]]

===EMDB===
[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/5335_summary.html 5335]: 9.7Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1888_summary.html 1888]: 16Å resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1640_summary.html 1640]: 25Å resolution map of Saccharomyces cerevisiae V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1590_summary.html 1590]: 17Å resolution map of Manduca sexta V-ATPase.

==References==

<references />

V-ATPase

2012-09-06T09:29:15Z

Gydo van Zundert: V-ATPase start page.

== Your Heading Here (maybe something like 'Structure') ==
<StructureSection load='3j0j' size='350' side='right' caption='Fitted structure of Thermus thermophilus V-ATPase, based on the EMD-5335 (PDB entry [[3j0j]])' scene=''>
Anything in this section will appear adjacent to the 3D structure and will be scrollable.

<scene name='V-ATPase/Adp/1'>TextToBeDisplayed</scene>
</StructureSection>

==Introduction==
V-ATPase are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolyzation of [[ATP]] in order to transport solutes.

==V-ATPase components==
The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolyzation of ATP, and the intermembrane V0 domain consisting of six different sub-units (a-f) and which transports the protons.
==Mechanism of rotation==

==V-ATPase structures==

===PDB===
[[3j0j]]

[[3a5c]]

[[3k5b]]

[[1r5z]]

===EMDB===
5335
==References==