Proteopedia - User contributions [en]

Dronpa

2008-12-02T14:05:09Z

Jamie LaFrance: Undo revision 804596 by Jamie LaFrance (Talk)

{{STRUCTURE_2iov | PDB=2iov | SCENE= }}

'''Dronpa''' is a [[:Category:Gfp-like protein|GFP]]-like photoswitchable protein. It was first designed by Ryoko Ando, Hideaki Mizuno, and Atsushi Miyawaki in 2004. The protein was named after the “Japanese term Dron, a ninja term referring to instant disappearance of the body, and pa, short for photo activation.”<ref name="webcite"> [http://www.brain.riken.jp/bsi-news/bsinews29/no29/research3e.html] (retrieved on November 30, 2008), </ref> Using a laser diode at 405nm the protein can be activated into its “bright” state, and using an argon laser at 480nm, the protein can be deactivated and turned into its “dark” state<ref name="webcite" />. Dronpa can be turned back and forth as often as necessary just by switching the wavelength of light that is focused onto it. This reversible ability to be able to switch back and forth between “dark” and “light” is what makes Dronpa unique.

=== Function===
Dronpa works by switching between the cis (bright state) to trans (dark state) position of a few amino acids in the chromophore center of the Beta-barrel structure. The amino acids that are key for this change are CYG Tripeptide in the chromophore Cys 62, Tyr 63, and Gly 64. There are also changes in conformation of four additional amino acids that are near the chromophore, Arg 66, Ser 142, Val 157, and His 193<ref> Biochem J. (2007) 402, 35-42 </ref>.

===References===
<references />
[[Category:Dronpa]]

Dronpa

2008-12-02T14:04:42Z

Jamie LaFrance: Undo revision 804597 by Jamie LaFrance (Talk)

{{STRUCTURE_2iov | PDB=2iov | scene='Dronpa/Dronpa/1' }}

'''Dronpa''' is a [[:Category:Gfp-like protein|GFP]]-like photoswitchable protein. It was first designed by Ryoko Ando, Hideaki Mizuno, and Atsushi Miyawaki in 2004. The protein was named after the “Japanese term Dron, a ninja term referring to instant disappearance of the body, and pa, short for photo activation.”<ref name="webcite"> [http://www.brain.riken.jp/bsi-news/bsinews29/no29/research3e.html] (retrieved on November 30, 2008), </ref> Using a laser diode at 405nm the protein can be activated into its “bright” state, and using an argon laser at 480nm, the protein can be deactivated and turned into its “dark” state<ref name="webcite" />. Dronpa can be turned back and forth as often as necessary just by switching the wavelength of light that is focused onto it. This reversible ability to be able to switch back and forth between “dark” and “light” is what makes Dronpa unique.

=== Function===
Dronpa works by switching between the cis (bright state) to trans (dark state) position of a few amino acids in the chromophore center of the Beta-barrel structure. The amino acids that are key for this change are CYG Tripeptide in the chromophore Cys 62, Tyr 63, and Gly 64. There are also changes in conformation of four additional amino acids that are near the chromophore, Arg 66, Ser 142, Val 157, and His 193<ref> Biochem J. (2007) 402, 35-42 </ref>.

===References===
<references />
[[Category:Dronpa]]

Dronpa

2008-12-02T14:04:00Z

Jamie LaFrance: Undo revision 804598 by Jamie LaFrance (Talk)

{{STRUCTURE_2iov | PDB=2iov | scene=Dronpa/Dronpa/1 }}

'''Dronpa''' is a [[:Category:Gfp-like protein|GFP]]-like photoswitchable protein. It was first designed by Ryoko Ando, Hideaki Mizuno, and Atsushi Miyawaki in 2004. The protein was named after the “Japanese term Dron, a ninja term referring to instant disappearance of the body, and pa, short for photo activation.”<ref name="webcite"> [http://www.brain.riken.jp/bsi-news/bsinews29/no29/research3e.html] (retrieved on November 30, 2008), </ref> Using a laser diode at 405nm the protein can be activated into its “bright” state, and using an argon laser at 480nm, the protein can be deactivated and turned into its “dark” state<ref name="webcite" />. Dronpa can be turned back and forth as often as necessary just by switching the wavelength of light that is focused onto it. This reversible ability to be able to switch back and forth between “dark” and “light” is what makes Dronpa unique.

=== Function===
Dronpa works by switching between the cis (bright state) to trans (dark state) position of a few amino acids in the chromophore center of the Beta-barrel structure. The amino acids that are key for this change are CYG Tripeptide in the chromophore Cys 62, Tyr 63, and Gly 64. There are also changes in conformation of four additional amino acids that are near the chromophore, Arg 66, Ser 142, Val 157, and His 193<ref> Biochem J. (2007) 402, 35-42 </ref>.

===References===
<references />
[[Category:Dronpa]]

Dronpa

2008-12-02T14:02:04Z

Jamie LaFrance:

{{STRUCTURE_2iov | PDB=2iov }}

'''Dronpa''' is a [[:Category:Gfp-like protein|GFP]]-like photoswitchable protein. It was first designed by Ryoko Ando, Hideaki Mizuno, and Atsushi Miyawaki in 2004. The protein was named after the “Japanese term Dron, a ninja term referring to instant disappearance of the body, and pa, short for photo activation.”<ref name="webcite"> [http://www.brain.riken.jp/bsi-news/bsinews29/no29/research3e.html] (retrieved on November 30, 2008), </ref> Using a laser diode at 405nm the protein can be activated into its “bright” state, and using an argon laser at 480nm, the protein can be deactivated and turned into its “dark” state<ref name="webcite" />. Dronpa can be turned back and forth as often as necessary just by switching the wavelength of light that is focused onto it. This reversible ability to be able to switch back and forth between “dark” and “light” is what makes Dronpa unique.

=== Function===
Dronpa works by switching between the cis (bright state) to trans (dark state) position of a few amino acids in the chromophore center of the Beta-barrel structure. The amino acids that are key for this change are CYG Tripeptide in the chromophore Cys 62, Tyr 63, and Gly 64. There are also changes in conformation of four additional amino acids that are near the chromophore, Arg 66, Ser 142, Val 157, and His 193<ref> Biochem J. (2007) 402, 35-42 </ref>.

===References===
<references />
[[Category:Dronpa]]

Dronpa

2008-12-02T14:00:39Z

Jamie LaFrance:

Dronpa

2008-12-02T14:00:01Z

Jamie LaFrance:

Dronpa

2008-12-02T13:53:07Z

Jamie LaFrance:

Dronpa

2008-12-02T13:51:55Z

Jamie LaFrance:

{{STRUCTURE_2iov | PDB=2iov | SCENE= }}

'''Dronpa''' is a [[:Category:Gfp-like protein|GFP]]-like photoswitchable protein. It was first designed by Ryoko Ando, Hideaki Mizuno, and Atsushi Miyawaki in 2004. The protein was named after the “Japanese term Dron, a ninja term referring to instant disappearance of the body, and pa, short for photo activation.”<ref name="webcite"> [http://www.brain.riken.jp/bsi-news/bsinews29/no29/research3e.html] (retrieved on November 30, 2008), </ref> Using a laser diode at 405nm the protein can be activated into its “bright” state, and using an argon laser at 480nm, the protein can be deactivated and turned into its “dark” state<ref name="webcite" />. Dronpa can be turned back and forth as often as necessary just by switching the wavelength of light that is focused onto it. This reversible ability to be able to switch back and forth between “dark” and “light” is what makes Dronpa unique.

=== Function===
Dronpa works by switching between the cis (bright state) to trans (dark state) position of a few amino acids in the chromophore center of the Beta-barrel structure. The amino acids that are key for this change are CYG Tripeptide in the chromophore Cys 62, Tyr 63, and Gly 64. There are also changes in conformation of four additional amino acids that are near the chromophore, Arg 66, Ser 142, Val 157, and His 193<ref>Anderson, et. all, 13006</ref>.

===References===
<references />
[[Category:Dronpa]]

Dronpa

2008-12-02T13:49:54Z

Jamie LaFrance:

{{STRUCTURE_2iov | PDB=2iov | SCENE= }}

'''Dronpa''' is a [[GFP]]-like photoswitchable protein. It was first designed by Ryoko Ando, Hideaki Mizuno, and Atsushi Miyawaki in 2004. The protein was named after the “Japanese term Dron, a ninja term referring to instant disappearance of the body, and pa, short for photo activation.”<ref name="webcite"> [http://www.brain.riken.jp/bsi-news/bsinews29/no29/research3e.html] (retrieved on November 30, 2008), </ref> Using a laser diode at 405nm the protein can be activated into its “bright” state, and using an argon laser at 480nm, the protein can be deactivated and turned into its “dark” state<ref name="webcite" />. Dronpa can be turned back and forth as often as necessary just by switching the wavelength of light that is focused onto it. This reversible ability to be able to switch back and forth between “dark” and “light” is what makes Dronpa unique.

=== Function===
Dronpa works by switching between the cis (bright state) to trans (dark state) position of a few amino acids in the chromophore center of the Beta-barrel structure. The amino acids that are key for this change are CYG Tripeptide in the chromophore Cys 62, Tyr 63, and Gly 64. There are also changes in conformation of four additional amino acids that are near the chromophore, Arg 66, Ser 142, Val 157, and His 193<ref>Anderson, et. all, 13006</ref>.

===References===
<references />
[[Category:Dronpa]]

Dronpa

2008-12-02T13:48:56Z

Jamie LaFrance: New page: {{STRUCTURE_2iov | PDB=2iov | SCENE= }} '''Dronpa''' is a GFP-like photoswitchable protein. It was first designed by Ryoko Ando, Hideaki Mizuno, and Atsushi Miyawaki in 2004. The...

{{STRUCTURE_2iov | PDB=2iov | SCENE= }}

'''Dronpa''' is a [[GFP]]-like photoswitchable protein. It was first designed by Ryoko Ando, Hideaki Mizuno, and Atsushi Miyawaki in 2004. The protein was named after the “Japanese term Dron, a ninja term referring to instant disappearance of the body, and pa, short for photo activation.”<ref name="webcite"> http://www.brain.riken.jp/bsi-news/bsinews29/no29/research3e.html (retrieved on November 30, 2008), </ref> Using a laser diode at 405nm the protein can be activated into its “bright” state, and using an argon laser at 480nm, the protein can be deactivated and turned into its “dark” state<ref name="webcite" />. Dronpa can be turned back and forth as often as necessary just by switching the wavelength of light that is focused onto it. This reversible ability to be able to switch back and forth between “dark” and “light” is what makes Dronpa unique.

=== Function===
Dronpa works by switching between the cis (bright state) to trans (dark state) position of a few amino acids in the chromophore center of the Beta-barrel structure. The amino acids that are key for this change are CYG Tripeptide in the chromophore Cys 62, Tyr 63, and Gly 64. There are also changes in conformation of four additional amino acids that are near the chromophore, Arg 66, Ser 142, Val 157, and His 193<ref>Anderson, et. all, 13006</ref>.

===References===
<references />
[[Category:Dronpa]]

User:Jamie LaFrance/Sandbox

2008-11-25T15:43:09Z

Jamie LaFrance:

Put text here.

<applet load='2iov' size='300' frame='true' align='right' caption='DRONPA' />

User:Jamie LaFrance/Sandbox

2008-11-25T15:28:24Z

Jamie LaFrance:

User:Jamie LaFrance/Sandbox

2008-11-25T15:27:30Z

Jamie LaFrance: New page: ==This is a placeholder== This is a placeholder text to help you get started in placing a Jmol applet on your page. At any time, click "Show Preview" at the bottom of this page to see how...

==This is a placeholder==
This is a placeholder text to help you get started in
placing a Jmol applet on your page. At any time, click
"Show Preview" at the bottom of this page to see how it goes.

Replace the PDB id after the STRUCTURE_ and after PDB= to load
and display another structure.

{{STRUCTURE_3cin | PDB=3cin | SCENE= }}

<applet load='2iov' size='300' frame='true' align='right' caption='DRONPA' />

User:Jamie LaFrance

2008-11-25T15:09:24Z

Jamie LaFrance:

I am a Ph.D. chemistry student at the University of Massachusetts-Amherst.

*[[User:Jamie LaFrance/Sandbox]]